响应面法优化黄精提取液制备工艺的研究

Study on Optimization of Preparation Process of Polygonatum sibiricum Extract by Response Surface Methodology

本实验通过对黄精采用蒸制的炮制方法,以感官评分和多糖含量为评价指标,经过工艺顺序筛选与确定、单因素考察及响应面实验,逐步确定并优化黄精提取液的制备工艺。确定工艺路线为鲜黄精→匀浆→蒸制→过滤→灭菌;最优工艺参数为蒸制时间61 min,蒸制温度92℃,匀浆时间105 s。此工艺具有实施可行性,可为后续的黄精多糖提取和开发提供参考依据。

In this experiment,the processing method of Polygonatum sibiricum was optimized based on the sensory score and polysaccharide content evaluation.Through the single factor investigation and response surface experiments,the optimal preparation process of Polygonatum sibiricum extract was determined as fresh Polygonatum sibiricum→homogenate→steaming→filtration→sterilization;the key optimal process parameters were steaming time 61 min,steaming temperature 92℃,homogenization time 105 s.This process is feasible and practical for the subsequent extraction and development of polysaccharide polysaccharides.