基于纳滤溶解扩散理论探索热毒宁注射液醇沉浓度对绿原酸分子态比例及传质行为的调控规律

Exploring the Regulation Rules of Ethanol Precipitation Concentration on Chlorogenic Acid Molecular State and Nanofiltration mass Transfer Behavior in Reduning Injection Based on the Solution-Diffusion Theory

目的研究热毒宁注射液醇沉浓度对绿原酸分子态比例及纳滤传质行为的调控规律。方法基于溶解-扩散效应,考察热毒宁注射液醇沉浓度对绿原酸分子态比例的影响,进而拟合传质系数与绿原酸浓度的相关性曲线,分析乙醇浓度对传质行为的调控规律。结果纳滤传质数学模型可用于热毒宁注射液醇沉中间体溶液中的绿原酸存在状态定量分析,醇沉浓度在60%~80%之间时,以分子态形式存在的绿原酸比例在22.21%~82.43%范围内,随着醇沉浓度升高,绿原酸的主要存在状态由离子态、缔合态向分子态过渡,迁移程度表现为80%醇沉>70%醇沉>60%醇沉。结论在高浓度乙醇溶液环境下,绿原酸单体、60%及70%醇沉中间体的纳滤分离行为可通过“溶解扩散-孔道位阻”效应协同调控分离,为有机溶液环境下的成分有序分离提供了研究思路。

OBJECTIVE To study the regulation rules of ethanol precipitation concentration on chlorogenic acid molecular state and nanofiltration mass transfer behavior in Reduning injection.METHODS Based on the solution-diffusion effect,the regulation rules of chlorogenic acid molecular state and nanofiltration mass transfer behavior were calculated and analyzed by mass transfer mathematical model in ethanol precipitation intermediates of Reduning injection.RESULTS The experiment showed that the nanofiltration mass transfer model was applied to the quantitative analysis of chlorogenic acid in the ethanol precipitation intermediate solution of Reduning injection.The proportion of chlorogenic acid in molecular form ranged from 22.21%to 82.43%when the concentration of ethanol precipitation was between 60%and 80%.With the increase of ethanol concentration,the main existing states of chlorogenic acid gradually changed from ionic state,associative state to molecular state.Meanwhile,the migration degree was 80%>70%>60%of ethanol precipitation concentration,respectively.CONCLUSION The nanofiltration separation behavior of chlorogenic acid in monomer solution,60%and 70%ethanol precipitation intermediates can be coordinated by the effect of"solution-diffusion and steric hindrance".This study provided a research road for the orderly separation of components in organic solution.