FSHR、pAKT及NF-kB在卵巢癌组织中的表达及与恶性程度的关系

Expression of FSHR,pAKT and NF-kB in ovarian cancer tissues and their relationship with the degree of malignancy

目的 探讨卵泡刺激素(FSHR)、磷酸化AKT(pAKT)及和核因子-kappaB(NF-kB)在卵巢癌组织中的表达及与恶性程度的关系。方法 选择2015年7月至2016年10月邯郸市中心医院妇二科卵巢癌患者89例为观察组;选择同期治疗的卵巢良性肿瘤患者68例为对照1组;选择同期非卵巢肿瘤女性51例为对照2组。采用免疫组织化学法及实时荧光PCR法检测三组FSHR、pAKT、NF-kB表达状况,采用Pearson相关性分析软件进一步分析FSHR、pAKT、NF-kB与卵巢恶性程度的关系。结果 各组FSHR、pAKT、NF-kB蛋白阳性率比较差异有统计意义(P<0.05)。三组FSHR、pAKT、NF-kB mRNA水平比较:观察组>对照1组>对照2组,差异有统计学意义(F=10.337、11.591、12.494,P<0.05)。卵巢癌组织在不同病理下FSHR、pAKT、NF-kB mRNA水平与组织学分类比较差异无统计意义(P>0.05);与肿瘤分期、病理分级、淋巴结转移及病理类型比较差异有统计意义(P<0.05)。Pearson相关性分析结果表明:卵巢癌组织中FSHR、pAKT、NF-kB水平与组织分类无相关性(P>0.05);与肿瘤分期、淋巴结转移呈正相关性(P<0.05);与病理分级呈负相关性(P<0.05)。ROC曲线结果表明:FSHR、pAKT、NF-kB联合检查在卵巢癌患者恶性程度中的预测灵敏度与特异度高于单一FSHR、pAKT、NF-kB检查(P<0.05)。结论FSHR、pAKT、NF-kB在卵巢癌组织中呈高表达,且与恶性程度有关,并影响患者预后,联合检测FSHR、pAKT、NF-kB对卵巢癌患者恶性程度评估具有重要意义。

Objective To investigate the expressions of follicle stimulating hormone(FSHR),phosphorylated AKT(p AKT)and nuclear factor-kappaB(NF-kB)in ovarian cancer tissues and their relationship with the degree of malignancy. Methods From July 2015 to October 2016,89 patients with ovarian cancer in the Second Department of Gynecology,Handan Central Hospital were selected as the observation group;68 patients with benign ovarian tumors treated during the same period were selected as the control group 1;51 women without ovarian tumors during the same period were selected for the control group 2. Immunohistochemistry and real-time PCR were used to detect the expression of FSHR,pAKT and NF-kB in the three groups. Pearson correlation analysis software was used to further analyze the relationship between FSHR,pAKT,NF-kB and ovarian malignancy. Results The positive rate of FSHR,pAKT and NF-kB protein in each group was significantly different(P<0.05). Comparison of FSHR,pAKT,NF-kB mRNA levels among the three groups:observation group > control group 1 > control group 2,the difference was statistically significant(F=10.337,11.591,12.494,P<0.05). There was no significant difference in FSHR,pAKT,NF-kBm RNA levels and histological classification in ovarian cancer tissues under different pathologies(P>0.05).Compared with tumor stage,pathological grade,lymph node metastasis and pathological type,there were significant differences(P<0.05). Pearson correlation analysis showed that there was no correlation between the levels of FSHR,p AKT and NF-k B in ovarian cancer tissue and tissue classification(P>0.05). There was a positive correlation with tumor stage and lymph node metastasis(P<0.05),and a negative correlation with pathological grade(P<0.05). The ROC curve results showed that the combined detection of FSHR,p AKT and NF-k B had higher sensitivity and specificity in predicting the malignancy of ovarian cancer patients than single FSHR,p AKT and NF-k B examinations(P<0.05). Conclusion FSHR,p AKT and NF-k B are highly expressed in ovarian cancer tissue,and are related to the degree of malignancy and affect the prognosis of patients. The combined detection of FSHR,p AKT and NF-k B is of great significance for evaluating the degree of malignancy in ovarian cancer patients.