具有钙磷离子可充性的抗菌和抗生物膜蛋白质黏附口腔粘接剂的研究

Development of a novel ion-rechargeable anti-caries dental bonding agent

目的开发一种新型口腔防龋粘接剂,具有钙和磷酸盐离子补充和重复释放,抗菌和抗蛋白质的三重功能,并首次研究粘接剂的钙磷离子补充和再释放能力。方法制作粘接剂样品。测量粘接剂牙本质粘接的剪切强度、钙磷离子的释放-补充-再释放能力、抗蛋白质的吸附效果、生物膜死活细菌和代谢活性分析。结果新型治疗性口腔粘接剂的牙本质粘接强度不受加入的抗蛋白质黏附、抗菌和钙磷离子补充成分影响(P>0.1)。与商品化粘接剂相比,新粘接剂的蛋白质吸附和代谢活性降低了10倍(P<0.05)。粘接剂于70天内持续释放高水平的钙磷离子,并且在进行离子补充后2周内再次释放高水平的离子。结论新型治疗性口腔粘接剂具备了长期的再矿化效果以及有效的杀菌和抗蛋白特性。

Objective To develop a novel anti-caries dental bonding agent with calcium and phosphate ion release capability and anti-bacterial function.Methods Adhesive testing samples were developed.Dentin shear bond strength,Ca and P ion release,recharge and re-release,and protein adsorption were determined.An in vitro human saliva microcosm biofilm model was used to analyze live/dead assay and metabolic activity of biofilms.Results Dentin bond strengths were not compromised by DMADDM,MPC and NACP(P>0.1).The new adhesive caused 10-fold drop in protein adsorption and metabolic activity,compared to a commercial adhesive(P<0.05).Large amounts of Ca and P ions were released in the test period of 70 days.With each recharge,the samples again had large amounts of ion release in the 2 week test period.The repeated ion re-releases showed no drop,with great potential for long lasting remineralization.Conclusions A new dental bonding agent was developed using NACP,DMADDM and MPC.It achieved long lasting recharge of remineralizing ions,together with anti-biofilm and protein-repellent functions to greatly reduce dental caries and protect the teeth.