慢性丙型肝炎病毒感染者血清自身抗体的检测分析

Detection and analysis of serum autoantibodies in patients infected with chronic hepatitis C virus

目的:分析慢性丙型肝炎病毒感染者血清自身抗体情况。方法:回顾性分析2020年1—12月该中心检验科45例慢性丙型肝炎病毒感染患者的临床资料,设为观察组,另选取同期45例健康体检者的临床资料,设为对照组。两组均进行血清自身抗体检测,比较两组血清自身抗体检测结果,观察组血清自身抗体阳性患者、阴性患者肝功能指标[丙氨酸氨基转移酶(ALT)、总胆红素(TBiL)、天门冬氨酸氨基转氨酶(AST)]水平,以及慢性丙型肝炎病毒感染者血清自身抗体阳性与肝功能指标水平的相关性。结果:观察组血清抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA)和总阳性率均高于对照组,差异有统计学意义(P<0.05);两组血清抗肝肾微粒体抗体(LKM)及抗Ro-52抗体阳性率比较,差异均无统计学意义(P>0.05);观察组血清自身抗体阳性患者ALT、AST、TBiL水平均高于阴性患者,差异有统计学意义(P<0.05);Spearman相关性分析显示,慢性丙型肝炎病毒感染者血清自身抗体阳性与肝功能ALT、AST、TBiL水平呈正相关(r>0,P<0.05)。结论:慢性丙型肝炎病毒感染患者血清自身抗体阳性率高于健康体检者,且血清自身抗体阳性与肝功能ALT、AST、TBiL水平呈正相关。

Objective:To analyze serum autoantibodies in patients infected with chronic hepatitis C virus.Methods:The clinical data of 45 patients infected with chronic hepatitis C virus in the clinical laboratory of the center from January to December 2020 were retrospectively analyzed,and they were set as observation group;the clinical data of 45 healthy subjects during the same period were also selected and they were set as control group.Serum autoantibodies were detected in both groups.The detection results of serum autoantibodies were compared between the two groups.The levels of liver function indexes[alanine aminotransferase(ALT),total bilirubin(TBiL),aspartate aminotransferase(AST)]in the patients with positive serum autoantibodies and the patients with negative serum autoantibodies in the observation group were analyzed.Further,the correlations among the levels of the serum autoantibody positivity and the liver function index levels in the patients infected with chronic hepatitis C virus were analyzed.Results:The serum ANA,SMA,AMA positive rates and the total positive rate of the observation group were higher than those of the control group,and the differences were statistically significant(P<0.05).There were no significant differences in the serum LKM and anti-Ro-52 antibody positive rates between the two groups(P>0.05).The levels of ALT,AST and TBiL in the patients with positive serum autoantibodies in the observation group were higher than those in the patients with negative serum autoantibodies,and the differences were statistically significant(P<0.05).Spearman correlation analysis showed that the positive serum autoantibodies in the patients infected with chronic hepatitis C virus were positively correlated with the levels of liver function ALT,AST and TBiL(r>0,P<0.05).Conclusions:The positive rate of serum autoantibodies in the patients infected with chronic hepatitis C virus is higher than that in the healthy people.Further,the positive serum autoantibodies are positively correlated with the levels of liver function ALT,AST and TBiL.