IL-8基因不同位点多态性与儿童幽门螺杆菌感染胃炎易感性的关联

Association between IL-8 gene polymorphism and susceptibility of Helicobacter pylori infection in children with gastritis

目的 探讨白细胞介素-8(IL-8)基因不同位点多态性对儿童幽门螺杆菌(Hp)感染胃炎易感性的影响。方法 选择海口市妇幼保健院儿科2017年12月-2020年12月门诊收治Hp感染患儿120例,将其分为Hp感染胃炎组(n=51)和非胃炎组(n=69)。采用限制性片段长度多态性聚合酶链反应检测IL-8+781 C>T、IL-8-251 T>A位点基因型分布。对Hp感染、炎性细胞浸润进行分级;采用酶联免疫吸附法检测IL-8水平。结果哈迪-温伯格检验结果显示胃炎组、非胃炎组患儿IL-8+781 C>T位点、IL-8-251 T>A位点基因型分布在理论值和实际值之间比较差异无统计学意义,具有群体代表性;胃炎组、非胃炎组患儿IL-8+781 C>T位点基因型分布比较差异无统计学意义,胃炎组IL-8-251 T>A位点AA基因型频率高于非胃炎组,TA基因型低于非胃炎组(P<0.05);IL-8-251 T>A位点不同基因型患儿炎症严重程度和血清IL-8水平比较差异有统计学意义(P<0.05),其中AA型重度炎症浸润、血清IL-8水平高于TT型和TA型(P<0.05),三组HP定量、滤泡比较差异无统计学意义。结论 IL-8-251 T>A位点携带A等位基因可能增加了本地区儿童对Hp胃炎易感性。

OBJECTIVE To explore the effect of interleukin-8(IL-8) gene polymorphisms at different sites on the susceptibility of Helicobacter pylori(Hp) infection in children with gastritis. METHODS A total of 120 children with H. pylori infection in the Department of Pediatrics of Haikou Maternal and Child Health Hospital from Dec 2017 to Dec 2020 were enrolled. They were divided into the Hp infection gastritis group(n=51) and non-gastritis group(n=69). Restriction fragment length polymorphism polymerase chain reaction was used to detect the genotype distribution of IL-8+781 C>T and IL-8-251 T>A loci. The Hp infection and inflammatory cell infiltration were graded and IL-8 levels were detected by enzyme-linked immunosorbent assay. RESULTS Hardy-Weinberg test showed that the theoretical value and the actual value of genotype distribution of IL-8+781 C>T locus and IL-8-251 T>A locus between the gastritis group and non-gastritis group were not significant, which can represent the population. There was no significant difference in the distribution of IL-8+781 C>T locus between the gastritis group and the non-gastritis group. The frequency of TA and AA genotypes at IL-8-251 T>A locus was significantly higher than that in the non-gastritis group, while TA genotypes were lower than that in the non-gastritis group(P<0.05). The severity of inflammation and serum IL-8 levels in IL-8-251 T>A locus children with different genotypes were significant(P<0.05). Severe inflammation infiltration and serum IL-8 levels in patients carrying AA type were significantly higher than those carrying TT type and TA type(P<0.05). There was no significant differences in HP quantitative and follicular comparison among the three groups. CONCLUSION Children carrying A allele in the IL-8-251 T>A locus may increase the susceptibility to HP gastritis.