迷迭香抗氧化成分乙醇浸提工艺优化及抗氧化性能研究

Study on optimization of ethanol extraction process and antioxidant properties of rosemary antioxidant components

为优化迷迭香抗氧化成分乙醇浸提提取工艺,并对其体外抗氧化活性进行评价。以提取物中抗氧化成分(鼠尾草酸+鼠尾草酚+迷迭香酸)得率为评价指标,探讨乙醇浓度、液料比和浸提时间对抗氧化成分得率的影响;采用响应面试验进行提取工艺优化,并研究冷冻干燥制得迷迭香粗提取物的体外抗氧化活性。得到最佳提取方案:乙醇浓度65%、液料比26:1 mL/g和浸提时间4天;迷迭香粗提取物具有较好的Fe3+还原力,其对ABTS、DPPH和OH自由基的IC50值分别为0.081,0.280,0.360 mg/mL。在此条件下迷迭香总抗氧化剂得率为5.418%。研究结果显示响应面模型具有较好的预测能力;迷迭香粗提取物具有一定的抗氧化活性。

To optimize the ethanol extraction process of antioxidant components from rosemary and evaluate its antioxidant activity in vitro,taking the yields of antioxidant components(carnosic acid+carnosol+rosmarinic acid)in the extract as the evaluation indexes,the effects of ethanol concentration,solid-liquid ratio and extraction time on the yields of antioxidant components were discussed.Meanwhile,the extraction process was optimized by response surface methodology,and the antioxidant activity of crude rosemary extract prepared by freeze-drying in vitro was studied.The optimum extraction conditions were as follows:the concentration of ethanol was 65%,the ratio of liquid to material was 26:1 mL/g,and the extraction time was 4 d;At the same time,rosemary crude extract has good Fe3+reducing power,and its IC50 values for ABTS,DPPH and OH radicals are 0.081 mg/mL,0.280 mg/mL and 0.360 mg/mL,respectively.Under these conditions,the total antioxidant yield of rosemary is 5.418%.The research results show that the response surface model has good prediction ability.The crude extract of rosemary has certain antioxidant activity.