响应面法优化黑皮鸡枞总黄酮提取工艺

Optimization of Extraction Process of Total Flavonoids from Oudemansiella raphanipes by Response Surface Methodology

以黑皮鸡枞为研究对象,考察了乙醇浓度、液料比、超声时间和温度4个因素,利用响应面法优化超声波辅助乙醇提取黑皮鸡枞中总黄酮,并通过NaNO-Al(OH)-NaOH法测定其吸光度,计算黑皮鸡枞总黄酮提取率。结果显示:乙醇浓度82%、液料比42∶1 (mL/g)、超声时间117 min、超声温度70℃为最佳提取工艺。该条件下,黑皮鸡枞总黄酮理论提取率为4.91767 mg/g,实际平均提取率为4.89 mg/g,响应估计值与实际值相对误差为0.57%,误差较小。提取工艺操作简单,提取率稳定,为黑皮鸡枞有效成分的高效提取利用与产品研发奠定了基础。

Taking Oudemansiella raphanipes as the research object, four factors of ethanol concentration, liquid-solid ratio, ultrasonic time and temperature are investigated.The ultrasonic-assisted ethanol extraction of total flavonoids from Oudemansiella raphanipes is optimized by response surface methodology, the absorbance is determined by NaNO-Al(OH)-NaOH method, and the extraction rate of total flavonoids from Oudemansiella raphanipes is calculated. The results show that the optimal process is the ethanol concentration of 82%, the liquid-solid ratio of 42∶1(mL/g), the ultrasonic time of 117 min and the ultrasonic temperature of 70 ℃. Under these conditions, the theoretical extraction rate of total flavonoids from Oudemansiella raphanipes is 4.91767 mg/g, the actual average extraction rate is 4.89 mg/g, the small relative error between the response estimated value and the actual value is 0.57%. The extraction process is simple and the extraction rate is stable, which has laid a foundation for the efficient extraction and utilization of the effective components of Oudemansiella raphanipes, and product research and development.