基于顶空-气相色谱-离子迁移谱法的酸橙枳实与甜橙枳实挥发性化学成分表征及鉴别研究

Characterization and identification of volatile components in Citrus aurantium L.and Citrus sinensis Osbeck.based on headspace-gas chromatography-ion mobility spectrometry

目的对酸橙枳实与甜橙枳实的挥发性化学成分进行表征以实现酸橙枳实、甜橙枳实及其混伪品的真伪鉴别。方法采用顶空-气相色谱-离子迁移谱法(headspace-gas chromatography-ion mobility spectrometry HS-GC-IMS)对酸橙枳实、甜橙枳实、枳实混伪品中的挥发性成分进行分析,筛选特征性的挥发性物质,分别建立酸橙枳实、甜橙枳实的特征性GC-IMS指纹图谱,正交偏最小二乘法判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)法用于验证筛选结果的合理性。结果共检测到174个峰,并对其中133个峰进行了定性分析,剩余41个化合物为首次在枳实中鉴定并报道。筛选得到酸橙特征性挥发性化合物共12个,甜橙枳实特征性挥发性化合物共8个,分别绘制酸橙枳实与甜橙枳实特征性GC-IMS指纹图谱,结合OPLS-DA结果可明显区分酸橙枳实与甜橙枳实、酸橙枳实与混伪品、甜橙枳实与混伪品。结论所建立的HS-GC-IMS特征性指纹图谱能够实现酸橙枳实与甜橙枳实的甄别以及两者与混伪品的鉴别,能够体现酸橙枳实与甜橙枳实的挥发性物质特征。

Objective To characterize the volatile constituents of Citrus aurantium L.and Citrus sinensis Osbeck.,realize the identification of Citrus aurantium L.,Citrus sinensis Osbeck.and their adulterants.Methods The volatile components of Citrus aurantium L.,Citrus sinensis Osbeck.and their adulterants were analyzed by headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS).The characteristic volatile substances were screened,and the characteristic GC-IMS fingerprints of Citrus aurantium L.and Citrus sinensis Osbeck.were established,respectively.Orthogonal partial least squares-discriminant analysis(OPLS-DA)method was used to verify the rationality of the results.Results A total of 174 peaks were detected,and 133 of them were qualitatively analyzed,the remaining 41 compounds were identified and reported for the first time.A total of 12 characteristic volatile compounds of Citrus aurantium L.were screened,and 8 characteristic volatile compounds of Citrus sinensis Osbeck.were obtained.The characteristic GC-IMS fingerprints of Citrus aurantium L.and Citrus sinensis Osbeck.were drawn,respectively,combined with OPLS-DA analysis,it could obviously distinguish between Citrus aurantium L.and Citrus sinensis Osbeck.,Citrus aurantium L.and its adulterants,Citrus sinensis Osbeck.and its adulterants.Conclusion The established HS-GC-IMS characteristic fingerprint can realize the identification of Citrus aurantium L.and Citrus sinensis Osbeck.,as well as the identification of the 2 and the adulterants,and can reflect the characteristics of volatile substances of Citrus aurantium L.and Citrus sinensis Osbeck..