新冠肺炎疫情期间山东省高校大学生心理应激反应与免疫球蛋白G N-聚糖的相关性

Association between psychological stress response and IgG N-glycan among the students of a university in Shandong Province during COVID-19 epidemic

目的探索山东省高校大学生疫情期间心理应激反应与免疫球蛋白G N-聚糖之间的相关性。方法根据纳入、排除标准选取疫情期间山东第一医科大学在读学生150例为研究对象。使用SRQ问卷调查大学生心理应激水平,采用超高液相色谱法进行血浆IgG N-聚糖的检测与分析。IgG N-聚糖的组间比较采用Mann-Whitney U秩和检验;使用多因素logistic回归分析在调整混杂因素后,探讨心理应激与IgG N-聚糖的关联性;采用Spearman秩相关进行IgG N-聚糖水平与心理应激水平的关联性分析。结果在心理应激高分组中GP4和GP8的水平相比正常组显著升高,G2水平显著降低,差异具有统计学意义(P<0.05)。心理应激程度与GP3、GP4、GP8和G0呈正相关关系,与GP12、GP14、GP17、GP18、G2、Stotal及S1呈负相关关系(P<0.05),整体表现为IgG半乳糖基化和唾液酸化修饰水平与心理应激程度呈负相关关系。结论山东某高校大学生疫情期间心理应激反应与IgG N-聚糖存在关联性。IgG N-聚糖所反映的半乳糖基化和唾液酸化修饰水平可能通过改变IgG的炎症效能影响心理应激反应的程度。

Objective:To explore the correlation between psychological stress response and immunoglobulin G(IgG)N-glycan among the students of a university in Shandong Province during the epidemic.Methods:According to the inclusion and exclusion criteria,150 students from Shandong First Medical University during the epidemic were selected as the research objects.SRQ questionnaire was used to investigate the psychological stress level of the college students,and ultra-high liquid chromatography was used to detect and analyze plasma IgG N-glycans.The Mann-Whitney U rank-sum test was used for comparison of IgG N-glycans between groups;multivariate Logistic regression analysis was used to explore the association between psychological stress and IgG N-glycans after adjusting for confounding factors;Spearman rank correlation correlation was used to analyze the correlation between IgG N-glycan level and psychological stress level.Results:Compared with the normal group,the levels of GP4 and GP8 in the high psychological stress group were higher,and the level of G2 was lower(P<0.05).The degree of psychological stress was positively correlated with GP3,GP4,GP8and G0,and negatively correlated with GP12,GP14,GP17,GP18,G2,Stotal and S1(P<0.05).On the whole,IgG galactosylation and salivary level of acidification modification were negatively correlated with the degree of psychological stress.Conclusion:There is a correlation between psychological stress response and IgG N-glycan among the students in a Shandong university during the epidemic.The level of galactosylation and sialylation reflected by IgG N-glycans may affect the degree of psychological stress response by altering the inflammatory potency of IgG.