某巡逻船聚集性诺如病毒疫情的病原基因特征分析

Etiological identification and phylogenetic analysis of a clustered epidemic caused by norovirus on a patrol boat

【目的】对浙江省岱山县2022年2月一起罕见的重组型诺如病毒暴发疫情进行病原学鉴定及基因分子特征分析。【方法】采用荧光定量聚合酶链反应(PCR)法对送检的8份肛拭样本进行诺如病毒病原学鉴定,用普通逆转录PCR(RT⁃PCR)对阳性样本基因扩增,用MEGA7.0及Simplot软件对扩增序列进行基因特征分析。【结果】送检的8份标本鉴定结果均为诺如病毒GⅠ型,基因特征分析为重组诺如病毒GⅠ.6[P11]亚型,重组位点位于ORF1⁃ORF2连接处。同源性最高(98.75%)的是2018年登录的GⅠ.6[P11]毒株序列(登录号:MT357995)。【结论】经病原学鉴定与基因特征分析,本次暴发疫情是由罕见重组型诺如病毒GⅠ.6[P11]亚型引起。

[Objective]To determine the pathogen and phylogenetic characteristics of an uncommon outbreak of recombinant norovirus infection in Daishan County in February 2022.[Methods]Fluorescence quantitative PCR was used to detect the norovirus in the eight anal swabs collected in the outbreak.In the positive samples,reverse transcription PCR were used to amplify the norovirus.Norovirus sequences were characterized by MEGA7 and Simplot.[Results]Norovirus GⅠwas identified in all eight anal samples.It was further determined to be recombinant norovirus GⅠ.6[P11],with the recombination site at the ORF1-ORF2 junction.The sequence had the highest nucleotide identity(98.75%)to a GⅠ.6[P11]strain collected in 2018(GenBank accession number MT357995).[Conclusion]According to the etiological identification and phylogenetic analysis,this outbreak is confirmed to be caused by the uncommon recombinant norovirus GⅠ.6[P11]in China.