摘要
目的 利用偶氮染色-光谱技术,研究并设计可满足牛乳中组胺、酪胺快速检测的方法。方法 样品经盐酸溶液提取,提取后的组胺、酪胺与偶氮试剂发生染色反应后10~15 min内使用光谱法检测。结果 组胺和酪胺的定量限均为10 mg/kg,满足当前国内外标准通常规定组胺最低为50 mg/kg的限量要求,回收率在90%左右,相对标准偏差在10%左右,结果符合食品理化分析的相关要求。结论 研制的检测方法具有快速、稳定、节约成本等特点,适用于牛乳中组胺、酪胺的快速检测。
Objective A rapid method for determination of histamine and tyramine in milk was designed and studied by azo dye-spectroscopy.Methods Histamine and tyramine in samples were extracted by hydrochloric acid solution,and dyed reaction was acted with azo reagent. Spectral method was used for detection within 10-15 min. External standard method was used for qualitative and quantitative analysis.Results The limits of quantification of histamine and tyramine were both 10 mg/kg,which met the minimum limit requirement of 50 mg/kg specified in the current domestic and foreign standards for histamine. The recovery rate was about 90% and the relative standard deviation was about 10%. The results met the relevant requirements of food physical and chemical analysis.Conclusion The detection method is fast,efficient,stable,reliable and cost saving. It is suitable for the rapid detection of histamine and tyramine in milk.
作者
陈雪营
樊静静
宋书锋
高洁
强维
蒋定国
贺巍巍
刘卿
杨大进
杨欣
CHEN Xueying;FAN Jingjing;SONG Shufeng;GAO Jie;QIANG Wei;JIANG Dingguo;HE Weiwei;LIU Qing;YANG Dajin;YANG Xin(ANPEL Laboratory Technologies(Shanghai)lnc.,Shanghai 201609,China;China National Center for Food Safety Risk Assessment,Beijing 100021,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2022年第5期943-946,共4页
Chinese Journal of Food Hygiene
基金
“十三五”国家重点研发计划(2018YFC1604304-02)。