低共熔溶剂辅助酶法制备稀有人参皂苷CK

Preparation of rare ginsenoside CK by DES assisted enzymatic method

针对在传统缓冲溶液中酶法转化人参皂苷Rb1产率低、中间物溶解度低、酶促转化速率慢等问题,通过低共熔溶剂辅助酶法对人参皂苷Rb1进行转化。考察了DES种类和质量浓度、pH值、温度、酶与底物质量比、反应时间对CK产率的影响,并通过响应面实验进行优化,得到转化反应的最优条件为pH=5.3的DES缓冲液中,反应温度为50℃,蜗牛酶与底物质量比为1∶1,反应36 h时,CK的产率可达87.92%。采用将人参皂苷溶解在DES溶剂中进行酶转化的方法,可较好地增加人参皂苷的溶解度,增强酶的二级结构,提高CK的产率,制备过程绿色环保、安全,适合工业化生产。

In view of the problems of low yield of enzymatic conversion of ginsenoside Rb1 in traditional buffer solution,low solubility of intermediates and slow enzymatic conversion rate,ginsenoside Rb1 was converted by deep eutectic solvent auxiliary enzymatic method.The effects of DES types and mass concentration,pH value,temperature,enzyme-substrate mass ratio and reaction time on CK yield were investigated,and the optimal conditions for the conversion reaction were obtained in DES buffer with pH=5.3,the mass ratio of snail enzyme to substrate was 1∶1,and the yield of CK could reach 87.92%at 50℃and the reaction for 36 h.The method of dissolving ginsenosides in DES solvent for enzymatic conversion was adopted,which can better increase the solubility of ginsenosides,enhance the secondary structure of enzymes,and improve the yield of CK,the preparation process is environmentally friendly,safe and suitable for industrial production.