虫草清肺胶囊在慢性支气管炎大鼠中的药效学研究

Pharmacodynamics Study of Chongcao Qingfei Capsule on Chronic Bronchitis in Rats

目的:探讨虫草清肺胶囊治疗慢性支气管炎大鼠的疗效及作用机制。方法:采用混合烟雾吸入法构建大鼠慢性支气管炎模型。60只大鼠随机均分为对照组、模型组、乙酰半胱氨酸组、虫草清肺胶囊(低、中、高剂量)组。每天灌胃给药1次,连续给药14 d。每天观察大鼠的一般情况,记录造模前及末次给药后各组大鼠的体质量。给药完成后,采用张力换能器、生物采集系统对大鼠的呼吸功能进行检测;采用血液细胞分析仪对大鼠支气管肺泡灌洗液白细胞进行计数及分类;采用酶联免疫吸附试验(ELISA)对大鼠肺组织肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)、IL-4、IL-10、P物质(SP)、血管活性肠肽(VIP)水平进行检测。结果:与模型组比较,虫草清肺胶囊可以显著改善慢性支气管炎大鼠倦怠、食欲减退、皮毛松弛无光泽、咳嗽、喘息、呼吸功能减退、体质量增长缓慢等特点(P<0.01)。此外,虫草清肺胶囊可以显著抑制慢性支气管炎大鼠支气管肺泡灌洗液中的白细胞、中性粒细胞、淋巴细胞,增加巨噬细胞(P<0.01);可以显著抑制慢性支气管炎大鼠肺组织的TNF-α、IL-8、SP水平,增加IL-4、IL-10、VIP水平(P<0.01)。结论:虫草清肺胶囊具有抗慢性支气管炎作用,并与调节神经肽介导的炎症有关。

Objective:To explore the pesticide effect and potential mechanism of Chongcao Qingfei capsule against chronic bronchitis in rats.Methods:Mixed smoke inhalation was used to establish a rat model of chronic bronchitis.Sixty rats were randomly divided into control group,model group,acetylcysteine group,and Chongcao Qingfei capsule(low,medium,and high dose)groups.Rats were orally administered with corresponding drug once a day for 14 consecutive days.The general situation and weight before modeling and after the last administration of rats were recorded.After administration,the respiratory function of rats was detected by tension transducer and biological acquisition system.Leukocytes in bronchoalveolar lavage fluid of rats were counted and classified by hematology analyzer.The levels of tumor necrosis factor-α(TNF-α),interleukin-8(IL-8),IL-4,IL-10,substance P(SP)and vasoactive intestinal peptide(VIP)in lung tissue of rats were analyzed by corresponding ELISA kits.Results:Compared with model group,Chongcao Qingfei capsule can significantly improve the characteristics of chronic bronchitis rats,such as burnout,anorexia,loose and dull fur,cough,wheezing,decreased respiratory function,slow weight growth and so on(P<0.01).In addition,Chongcao Qingfei capsule can significantly inhibit the increase of leukocytes,neutrophils and lymphocytes and the decrease of macrophages in bronchoalveolar lavage fluid of chronic bronchial rats(P<0.01).Meanwhile,Chongcao Qingfei capsule can also significantly inhibit the levels of TNF-α,IL-8,and SP and increase the IL-4,IL-10,and VIP levels in lung tissues of rats with chronic bronchitis(P<0.01).Conclusion:Chongcao Qingfei capsule shows the anti-chronic bronchitis effect,which is related to the regulation of neuropeptide-mediated inflammation.