基于脂质组学的绞股蓝总苷调血脂作用研究

Effect of gypenosides on regulating blood lipid based on lipidomics

目的 基于超高效液相色谱-四极杆-飞行时间质谱的脂质组学技术研究绞股蓝总苷对高脂血症大鼠脂质代谢的影响。方法 采用高脂饲料制备高脂血症大鼠模型,通过血清血脂指标和肝脏组织病理学评价绞股蓝总苷的调血脂活性,应用超高效液相色谱-四极杆-飞行时间质谱技术检测血清和肝脏的脂质代谢物,结合主成分分析、正交偏最小二乘-判别分析、火山图筛选差异脂质,明确绞股蓝总苷干预前后血清和肝脏中脂质变化。结果 绞股蓝总苷显著调节高脂血症大鼠的血脂(P<0.05、0.01)。从血清和肝脏中分别筛选了17、17个差异脂质,主要为三酰甘油、磷脂酰胆碱、溶血磷脂酰胆碱类。给予绞股蓝总苷干预后,筛选的差异脂质均有回调至正常的趋势。结论 绞股蓝总苷的调血脂作用确切,可能与调节三酰甘油、甘油磷脂、不饱和脂肪酸代谢有关。

Objective To study the antihyperlipidemic effects of gypenosides in hyperlipidemic rats using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS)-based lipidomics. Methods A hyperlipidemic rat model was induced by high-fat diet. The antihyperlipidemic effects of gypenosides were confirmed by serum lipid index and liver pathological examination. Lipid metabolites in serum and liver were detected by UPLC-Q-TOF/MS method. The MS data were processed by principle component analysis, orthogonal partial least squared discriminant analysis and volcanic map analysis to screen the differential lipids. Results Gypenosides significantly regulated the levels of serum lipids in hyperlipidemic rats(P < 0.05, 0.01).A total of 17, 17 differential lipids, mainly belonging to triglyceride, phosphatidylcholine and lysophosphatidylcholine, were screened and identified from the serum and liver, respectively. Those differential lipids showed a tendency of callback to normal level after gypenosides intervention. Conclusion Gypenosides possessed significant antihyperlipidemic activity. The mechanism may be related to the regulation of triglyceride, glycerophospholipid and unsaturated fatty acid metabolism.