林下三七不同部位黄酮类物质差异分析

Variance Analysis of Flavonoids in Different Parts of Panax notoginseng

为了解林下三七中药材不同部位的黄酮类,以林下三七中地下部分根和地上部分茎、叶、果为材料,用上调LC-MS/MS技术检测其黄酮类活性物质。应用MVDB和代谢信息公共数据库对三七不同部位黄酮类物质的差异代谢物进行PCA分析、OPLS-DA分析、样本重复相关评估等,通过KEGG数据库注释差异代谢物参与的代谢途径,从中筛选出差异显著的差异代谢物以及在不同部位的差异情况。结果表明:三七4个部位共检测出149个黄酮类次级代谢产物,三七中黄酮类数量排序为S(茎)>F(果)>L(叶)>R(根),叶、果、茎较根中含有更多的黄酮类物质;以地下部分根为对照组,比较了其与地上部分茎、叶、果的差异,其中F vs.R组中筛选出上调6个(没食子儿茶素、扁蓄苷、圣草酚-3′-O-葡萄糖苷等),下调14个(槲皮素、天竺葵素-3-O-葡萄糖苷、圣草酚-8-C-葡萄糖苷等);L vs.R组中筛选出上调2个(异鼠李素-3-O-阿拉伯糖苷、没食子儿茶素没食子酸酯),下调18个(芍药花素-3-O-葡萄糖苷、山奈素-3-O-葡萄糖醛酸苷、杨梅素-3-O-半乳糖苷等);S vs.R组中筛选出上调4个(4′,5,7-三羟基-3′,6-二甲氧基黄酮(棕矢车菊素)、扁蓄苷、山奈酚-7-O-葡萄糖苷等),下调16个(槲皮素、山奈素-3-O-葡萄糖醛酸苷、杨梅素-3-O-半乳糖苷等);叶、果、茎中含有大量与根中不同的特有差异次级代谢产物,如天竺葵素-3-O-葡萄糖苷、牵牛花素-3-O-芸香糖苷、异鼠李素-3-O-(2′′-O-木糖基)葡萄糖苷等可作为区别根的标志性差异物。三七不同部位的黄酮类活性物质存在较大差异,黄酮类含量以叶、果、茎中相对含量多,根中黄酮类相对含量相较其他部位少。

Using underground partial roots and aboveground stems,leaves and fruits of Panax notoginseng as materials.The difference of flavonoids in different parts of P.notoginseng were detected by LC-MS/MS technology.PCA analysis,OPLS-DA analysis and repeated correlation evaluation of samples were carried out for detected metabolites by using the MVDB and metabolism database.Then,KEGG database was used to annotate the metabolic pathways involved in differential metabolites to screen out differential metabolites and analyze their differences in different parts.The findings indicated that a total of 149 differential metabolites were detected in 4 sites,the number of flavonoids in P.notoginseng is S(stem)138>F(fruit)134>L(leaf)127>R(root)120.Leaves,fruits,and stems contain more flavonoids than roots;using the underground partial roots as the control,comparing the differences with aerial stems,leaves and fruit.Among them,in the F vs R group,6 were selected(epigallocatete catechin,planstorage side,copol-3′-O-glucoside,etc.),down-regulated 14(quercetin,geranin-3-O-glucoside,phenol-8-C-glucoside,etc.);in the L.vs.R group,up-regulated 2(isrhamin-3-O-arabinoglyside,antochatechin gallate),down-regulated 18(peony anthocyanin-3-O-glucoside,sanannin-3-Oglucuronside,myricetin-3-O-galactoside,etc.);in the S vs.R group,4 genes were up-regulated(4′,5,7-trihydroxyl3′,6-dimethoxyflavones(palm vecuthrin),flat storage side,kaempferol-7-O-glucoside,etc.),down-regulated 16(quercetin,sanonesin-3-O-glucuronide,myricetin-3-O-galactoside,etc.);leaves,fruits and stems contain a large number of different secondary metabolites with different characteristics and roots.For example,geranium-3-Oglucoside,morning glory-3-O-rutcoside,isrhamin-3-O-(2′′-O-xylosyll)glucoside can be used as landmark differences differentiating roots.The flavonoids active substances in different parts of P.notoginseng varied greatly,the relative content of flavonoids in different tissue parts of Panax notoginseng was higher in leaves,fruits and stems,and lower in roots than other parts.