GPR120、SCC-Ag、PD-1在非小细胞肺癌中的表达及其与临床特征、预后的关系

Expression of GPR120, SCC-Ag and PD-1 in Non-small Cell Lung Cancer and the Relationship with Clinical Characteristics and Prognosis

目的:探究G蛋白耦联受体120(GPR120)、鳞状细胞癌抗原(SCC-Ag)、程序性死亡受体1(PD-1)在非小细胞肺癌中的表达及其与临床特征、预后的关系。方法:选取2018年1月1日—2019年12月31日新疆生产建设兵团第一师医院收治的经手术治疗的81例非小细胞肺癌患者。分析所有患者临床特征(性别、年龄、肿瘤直径、分化程度、临床分期、淋巴结转移、累及程度)及预后情况,检查所有患者癌组织、癌旁组织GPR120 mRNA、SCC-Ag、PD-1 mRNA。比较所有患者癌组织、癌旁组织GPR120 mRNA、SCC-Ag、PD-1 mRNA。比较不同临床特征患者癌组织GPR120 mRNA、SCC-Ag、PD-1mRNA。比较存活组及死亡组GPR120 mRNA、SCC-Ag、PD-1 mRNA。分析GPR120 mRNA与SCC-Ag、PD-1 mRNA的关系。分析GPR120 mRNA、SCC-Ag、PD-1 mRNA对患者预后的预测价值。结果:癌组织中的GPR120 mRNA、SCC-Ag、PD-1m RNA均高于癌旁组织,差异有统计学意义(P<0.05)。低分化、Ⅲ~Ⅳ期、有淋巴结转移、累计肌层患者癌组织GPR120m RNA、SCC-Ag、PD-1 mRNA均高于中高分化、Ⅰ~Ⅱ期、无淋巴结转移、未累计肌层患者,差异有统计学意义(P<0.05)。81例患者随访6个月,22例死亡,59例存活。死亡组GPR120 mRNA、SCC-Ag、PD-1 mRNA均明显高于存活组,差异有统计学意义(P<0.05)。Pearson结果显示,GPR120 mRNA与SCC-Ag呈正相关(r=0.368,P=0.001);GPR120 mRNA与PD-1 mRNA呈正相关(r=0.314,P=0.004);SCC-Ag与PD-1 mRNA呈正相关(r=0.295,P=0.007)。ROC曲线分析,三项联合诊断AUC值高于GPR120 mRNA、SCC-Ag、PD-1 mRNA单项诊断。结论:在非小细胞肺癌中GPR120、SCC-Ag、PD-1m RNA呈高表达状态,与患者分化程度、临床分期、淋巴结转移、累及程度相关,且与患者预后相关,三项联合检测对非小细胞肺癌患者预后存活、死亡的判断价值较高。

Objective:To explore the expression of G protein coupled receptor 120(GPR120),squamous cell carcinoma antigen(SCC-Ag)and programmed death receptor 1(PD-1)in the non small cell lung cancer and its relationship with clinical characteristics and prognosis.Method:A total of 81 patients with non-small cell lung cancer who received surgical treatment in the First Division Hospital of Xinjiang Production and Construction Corps from January 1,2018 to December 31,2019 were selected.Clinical characteristics(gender,age,tumor diameter,degree of differentiation,clinical stage,lymph node metastasis,and degree of involvement)and prognosis condition of all patients were analyzed,and GPR120 mRNA,SCC-Ag,and PD-1 mRNA in cancer and para-cancer tissues of all patients were examined.The GPR120 mRNA,SCC-Ag and PD-1 mRNA in cancer tissues and adjacent cancer tissues of all patients were compared.The GPR120 mRNA,SCC-Ag and PD-1 mRNA in cancer tissues of patients with different clinical characteristics were compared.GPR120 mRNA,SCC-Ag,PD-1 mRNA were compared between the survival group and the death group.The relationship between GPR120 mRNA and SCC-Ag and PD-1 mRNA was analyzed.The predictive value of GPR120 mRNA,SCC-Ag and PD-1 mRNA for the prognosis of patients was analyzed.Result:The GPR120 mRNA,SCC-Ag and PD-1 mRNA in cancer tissues were higher than those in adjacent cancer tissues,and the differences were statistically significant(P<0.05).The GPR120 mRNA,SCC-Ag and PD-1 mRNA in cancer tissues of patients with low differentiation,stageⅢtoⅣ,lymph node metastasis and accumulated myometrium were higher than those of patients with medium and high differentiation,stageⅠtoⅡ,no lymph node metastasis and no accumulated myometrium,and the differences were statistically significant(P<0.05).Eighty-one patients were followed up for 6 months,22 patients died and 59 patients survived.GPR120 mRNA,SCC-Ag and PD-1 mRNA in the death group were significantly higher than those in the survival group,and the differences were statistically significant(P<0.05).Pearson results showed that GPR120 mRNA was positively correlated with SCC-Ag(r=0.368,P=0.001).GPR120 mRNA was positively correlated with PD-1 mRNA(r=0.314,P=0.004).SCC-Ag was positively correlated with PD-1 mRNA(r=0.295,P=0.007).ROC curve analysis showed that the AUC value of the three combined diagnoses was higher than that of GPR120 mRNA,SCC-Ag and PD-1 mRNA alone.Conclusion:GPR120,SCC-Ag and PD-1 mRNA are highly expressed in non-small cell lung cancer,which are correlated with the degree of differentiation,clinical stage,lymph node metastasis and extent of involvement,as well as the prognosis of patients.The combined detection of the three factors has a high value in judging the survival and death of patients with non-small cell lung cancer.