少阴病(心肾阳虚证)“欲解时”PGC-1α通路蛋白表达的节律变化及四逆汤干预作用的研究

A study on the rhythm changes of PGC-1α pathway protein expression in Shaoyin disease(cardiac-kidney yang deficiency)and the intervention effect of Sini Decoction

目的通过检测心肾阳虚证模型大鼠在“欲解时”及其对应时段心肌组织能量代谢通路关键蛋白的变化,探讨少阴病“欲解时”的时间治疗学意义。方法SD大鼠随机分为欲解时正常组、非欲解时正常组、欲解时模型组、非欲解时模型组、欲解时药物组、非欲解时药物组。造模及给药结束后,分别于少阴病“欲解时”及“非欲解时”取心肌组织,连续6d。Western Blot检测欲解时和非欲解时实验大鼠心肌组织PGC-1α、NRF-1、TFAM蛋白水平。结果模型组和药物组PGC-1α、NRF-1、TFAM的蛋白表达大多为欲解时高于非欲解时;药物组心肾阳虚证大鼠的PGC-1α、NRF-1、TFAM通路蛋白表达量较模型组高。结论欲解时PGC-1α、NRF-1、TFAM通路的蛋白表达量较非欲解时高。四逆汤对心肾阳虚证模型大鼠PGC-1α、NRF-1、TFAM通路蛋白的表达有上调作用。

Objective In this study,this study explored the temporal therapeutic significance of ShaoYin disease(cardiac-kidney-yang deficiency)by using the changes in key proteins expressions of key proteins of the energy metabolism pathway of myocardial tissue during the"Yujie time"and corresponding periods in the model of cardiac-kidney-yang deficiency.Methods SD rats are randomly divided into normal group at"Yujie time",normal group at"Non-Yujie Time",model group at"Yujie Time",model group at"Non-Yujie Time",drug group at"Yujie Time",and drug group at"Non-Yujie Time".After the end of molding and administration,the myocardial tissue was taken from"Yujie Time"and"Non-Yujie Time"for 6 days.Western Blot detected the levels of PGC-1α,NRF-1,TFAM protein in experimental rat myocardial tissue during the Yujie Time and Non-Yujie Time.Results The expression of proteins in PGC-1α,NRF-1 and TFAM in the model group and drug group was higher than that in Non-Yujie Time,and the expression of PGC-1α,NRF-1 and TFAM pathway proteins in rats with cardiovascular and renal yang deficiency in the drug group was higher than that in the model group.Conclusion The expression of protein in PGC-1α,NRF-1 and TFAM pathways was higher in Yujie Time than Non-Yujie Time.The Sini Decoction had an upregulating effect on the expression of PGC-1α,NRF-1 and TFAM pathway proteins in rats with cardiac-kidney yang deficiency model.