小米制品对番泻叶致小鼠腹泻的缓解作用

Alleviating Effect of Millet Products on Diarrhea Induced by Senna Leaf in Mice

探讨不同小米制品对番泻叶致小鼠腹泻的缓解作用。84只昆明小鼠随机分为7组:正常对照组、模型组、阳性对照组、发酵小米组(6.16 g/kg)、发酵米糠组(2.05 g/kg)、小米粥油组(10.27 g/kg)、米糠粥油组(10.27 g/kg)。采用番泻叶建立小鼠腹泻模型,造模7 d后,除模型组外,其它各组开始为期7 d的治疗,以腹泻指数、脏器指数、血清炎症因子、分泌型免疫球蛋白A(secretory immunoglobulin A,SIgA)水平为指标,并通过小肠切片观察来评价小米制品对腹泻小鼠的影响。结果表明,与模型组相比,小米制品组第13 d小鼠体重显著增加(P<0.05),腹泻指数显著降低(P<0.05),发酵米糠、发酵小米和小米粥油组血清炎症因子含量显著降低(P<0.05),肠粘膜分泌型免疫球蛋白A含量显著升高(P<0.05),小肠组织切片观察完整程度较好。小米制品中发酵米糠、发酵小米和小米粥油对番泻叶诱导小鼠腹泻有一定的缓解作用,但治疗作用机制及肠道菌群变化仍有待进一步探讨。

To study the alleviating effect of different millet products on diarrhea caused by senna leaf in mice.Eighty-four KM mice were randomly divided into 7 groups:Normal control group,model group,positive control group,fermented millet group(6.16 g/kg),ferment millet bran group(2.05 g/kg),millet gruel oil group(10.27 g/kg),and millet bran porridge oil group(10.27 g/kg).Senna leaf was used to establish a diarrhea model in mice.After 7 days of modeling,except for the model group,the other groups were treated for 7 days.The diarrhea index,viscera index,serum inflammatory factors,secretory immunoglobulin A(SIgA)levels,and small intestine section observation were used to evaluate the effects of millet products on diarrhea mice.Compared with the model group,the weight of mice in the millet product group increased significantly on the 13th day(P<0.05),the diarrhea index was significantly decreased(P<0.05),the contents of serum inflammatory factors in ferment millet bran group,fermented millet group,and millet porridge oil group were significantly decreased(P<0.05),the content of secreted immunoglobulin A in intestinal mucosa was significantly increased(P<0.05),small intestine tissue section observation integrity was good.Fermented millet bran,fermented millet,and millet porridge oil in millet products could alleviate diarrhea induced by senna leaf in mice,but the therapeutic mechanism and the changes of intestinal flora in them remain to be further explored.