EMS诱变创制水稻抗乙酰辅酶A羧化酶抑制剂类除草剂种质

EMS mutagenesis to create rice anti-acetyl-CoA carboxylase inhibitor-herbicide germplasm

创制非转基因抗除草剂水稻种质资源对于稻田杂草防控具有重要价值。本研究以甲基磺酸乙酯(EMS)水溶液诱变镇糯19水稻种子,获得1株能稳定遗传的可耐受乙酰辅酶A羧化酶(ACCase)抑制剂类除草剂高效盖草能的M 3代水稻幼苗(突变体)。分别扩增镇糯19野生型和突变体的基因组DNA并进行测序和序列比对,发现突变体ACCase基因的开放阅读框(ORF)的第5374位碱基发生了点突变,导致编码的第1792位氨基酸由异亮氨酸突变为亮氨酸。镇糯19野生型和突变体分蘖盛期大田喷施3种田间推荐剂量的ACCase抑制剂类除草剂后农艺性状调查结果表明突变体对高效盖草能、精喹禾灵和唑啉草酯抗性明显高于野生型。本研究获得了能稳定遗传的非转基因抗ACCase抑制剂类水稻新种质,具有一定的应用价值,为抗除草剂水稻育种提供了种质资源。

Cultivating non-transgenic herbicide-resistant rice germplasm resources is of great value for weed control in rice fields.In this study,Zhennuo 19 rice seeds were mutagenized by ethyl methyl sulfonate(EMS)solution,and a M 3 generation of rice seedlings with stable inheritance and tolerance to acetyl-CoA carboxylase(ACCase)inhibitor herbicides were obtained.The genomic DNAs of wild-type and the mutant were amplified and sequenced respectively.It was found that there was a point mutation at the 5374 th base of the open reading frame of the resistant rice ACCase gene,resulting in a mutation of the encoded 1792 th amino acid from isoleucine to leucine.Three kinds of ACCase inhibitor herbicides were sprayed in the field and the agronomic traits were analyzed.The results showed that the resistance of the mutant to haloxyfop-R-methyl,quizalofop-P-ethyl and pinoxaden was significantly higher than that of wild type.In this study,a new non-transgenic rice germplasm with ACCase inhibitor resistance was obtained,which had certain application value and could provide germplasm resources for herbicide-resistant rice breeding.