病毒保存液相关性能研究

Study on the performance of virus preservation solution

目的研究病毒保存液用于病毒保存和病毒灭活效果的相关性能。方法采用病毒滴度测定和PCR测定方法,对新研制的病毒保存液用于病毒保存和病毒灭活效果相关性能进行测定。结果灭活型病毒保存液保存的病毒在2 h后完全失活。非灭活型病毒保存液保存的病毒活性能保存6 h以上。两种保存液的核酸保存能力在37℃静置达4 h,在室温(28℃)静置达8 h,在4℃静置达24 h。结论该病毒保存液的病毒保存效果、核酸保存力和灭活效果良好,能满足病毒试验操作需求。

Objective To study the performance of virus preservation solution for virus preservation and virus inactivation.Methods The virus titer and PCR assay methods were used to evaluate the performance of the newly developed virus preservation solution for virus preservation and virus inactivation.Results The virus preserved in the inactivated virus preservation solution was completely inactivated after 2 h.The virus activity preserved in the non-inactivated virus preservation solution could be preserved for more than 6 h.Under the static condition,the nucleic acid preservation ability of the two preservatives was 4 h at 37℃,8 h at room temperature(28℃),and 24 h at 4℃.Conclusion The virus preservation solution has good performance in virus preservation,nucleic acid preservation ability,and inactivation effect,which can meet the requirements for virus experimental operations.