利用RhD血型设计和模拟常规输血血清学教学试验

Design and simulate routine serologic training experiments in blood transfusion using RhD blood group

目的利用RhD血型设计和模拟输血学技术中的常规血清学试验,以解决试验教学中阳性标本不易获得的问题。方法利用RhD阳性红细胞、RhD阴性红细胞和抗-D试剂设计并模拟输血学技术中的酶处理试验,吸收放散试验,抗体鉴定试验和凝聚胺法交叉配血试验。结果木瓜蛋白酶处理红细胞可使RhD阳性红细胞与IgG抗-D反应出现肉眼可见的凝集;用RhD阳性红细胞和IgG抗-D成功模拟了吸收放散试验;通过创建不同的鉴定谱和鉴定细胞组合,成功模拟抗-Jk^(a)和抗-Fy^(a)的抗体鉴定;利用RhD阴性和阳性红细胞与IgG抗-D反应,成功模拟了凝聚胺法交叉配血相合与不相合的两种情况。结论利用RhD血型可以模拟输血常用的大部分血清学试验,可用于输血血清学试验教学。

Objective To design and simulate routine serological experiments in transfusion techniques using RhD blood group,so as to solve the problem of difficult to obtain positive specimens in experimental teaching.Methods RhD positive red blood cells,RhD negative red blood cells and anti-D reagent were used to design and simulate the enzyme treatment experiment,absorption and elution test,antibody identification experiment and cross matching experiment of polybrene technology in transfusion techniques.Results Papain treatment of red blood cells made the agglutination of RhD positive red blood cells and IgG anti-D visible.Absorption and elution test were successfully simulated with RhD positive red blood cells and IgG anti-D reagent.The antibody identification of anti-Jk^(a) and anti-Fy^(a) was successfully simulated by creating different identification panel and panel cells made by RhD positive cells and negative cells.Cross matching test of polybrene method can also be simulated using RhD negative and positive red blood cells and IgG anti-D reagent.Conclusion RhD blood group can be used to simulate most of the routine serological experiments of blood transfusion,which can be used in the serological laboratory teaching of transfusion.