一测多评法同时测定广西桑叶及其炮制品中5种活性成分含量

Simultaneous Determination of 5 Active Ingredients in Mulberry Leaves from Guangxi Province by QAMS

目的:建立HPLC一测多评法同时测定广西桑叶及其炮制品中5种活性成分含量,分析炮制对其影响。方法:以芦丁为参照物,建立绿原酸(A)、异槲皮苷(B)、紫云英苷(C)和槲皮素(D)的相对校正因子,计算各成分含量。对外标一点法和一测多评法测定的结果进行比较。结果:采用校正因子计算的含量值与外标法实测值之间无显著差异,经炮制后桑叶绿原酸、芦丁、紫云英苷和异槲皮苷含量升高,在蜜量相同情况下:蜜烘桑叶>蜜炙桑叶>蜜蒸桑叶>炒桑叶>桑叶;槲皮素含量:桑叶>炒桑叶>蜜烘桑叶>蜜炙桑叶>蜜蒸桑叶。结论:以芦丁为参照物建立的一测多评法可用于桑叶质量评价,加热和蜜制均对桑叶化学成分产生了一定影响,以蜜烘法最为明显。

Objective:To establish a method and validate its feasibilities for quality evaluation of Mulberry leaves and its processed products by quantitative analysis of multi-components with single maker(QAMS),and analyze the effect on the content of the five active ingredient before and after processing.Methods:Rutin was chosen as the internal reference substances, the relative correction factors(RCFs) of chlorogenic acid(A),isoquercitrin(B),astragalin(C),and quercetin(D) to rutin(S) were established.The contents of these five active ingredients were determined by external standard method and QAMS method.The comparison of the quantitative results between the above methods.Results:No significant differences were found in the quantitative results of three ingredients by external standard method and quantitative analysis of multi-components(QAMS) method.The contents of chlorogenic acid, rutin, venetin and isoquercitrin of Mulberry leaves increased after processed by different method, and with the same amount of honey it showed the same change trend as follows: honey-baked Mulberry leaves>honey roasted Mulberry leaves> honey-steamed Mulberry leaves>fried Mulberry leaves>Mulberry leaves;quercetin content was Mulberry leavesfried Mulberry leaves>honey-baked Mulberry leaves>honey-roasted Mulberry leaves>honey-steamed Mulberry leaves.Conclusion:The RCFs established in the QAMS methods with rutin as the internal reference substances is accurate and feasible.It can be used to control the quality of Mulberry leaves.Both heating and honey preparation have a certain effect on the active ingredients in Mulberry leaves from Guangxi Province, and honey roasted preparation was more obvious.