异常环境温度对海蜇足囊生殖影响的比较研究

The effect of abnormal ambient temperature on podocyst reproduction of Rhopilema esculentum

为了探索海蜇无性繁殖育种的可能性,扩增海蜇螅状体数量,提高海蜇足囊生殖效率,本文以30℃高温处理作为对照,比较研究了变温和循环变温2种不同温度处理条件下海蜇螅状体足囊生殖情况。结果表明:变温对比实验中,高温组足囊形成速率为0.396±0.038 ind./d,变温组足囊形成速率为0.060±0.015 ind./d,高温组约为变温组的6.6倍。循环变温对比实验中,高温组足囊形成速率为0.204±0.027 ind./d,循环变温组足囊形成速率为0.183±0.027 ind./d,高温组足囊形成速率略高于循环变温组。本研究中足囊萌发率并不太高,高温处理组足囊萌发率最高可达10.8%,但能够在一定程度上提升螅状体种群的数量。相对于30℃以下降温与升温处理而言,30℃左右高温处理可将海蜇螅状体足囊形成速率提高5.6倍,显著地提高了海蜇螅状体的足囊生殖效率及其种群数量。本文研究表明,适宜的高温处理手段能够显著地提高其足囊生殖效率,有望作为海蜇螅状体种群无性繁殖数量扩增的有效手段。

In order to exploit the possibility of asexual reproduction and breeding of Rhopilema esculentum,increase the stocks number of polyps and improve the podocyst reproduction efficiency of R.esculentum,we used 30℃(high temperature,HT)as the control and studied the effect of two different temperature treatments,variation treatment(VT)and loop variation treatment(LVT),on the podocyst reproduction of R.esculentum.The results showed that the ratio of podocyst production(RPP)is 0.396±0.038 ind./d,0.060±0.015 ind./d in HT and VT groups,respectively.The RPP of HT is 6.6 times of that of VT.The RPP is 0.204±0.027 ind./d,0.183±0.027 ind./d in HT and LVT groups,respectively.The RPP of HT is slightly higher than that of LVT.The ratio of podocyst excyst(RPE)in this study is not too high and the highest RPE is 10.8%in HT,indicating that the stocks number of polyps may be increased to some extent.Compared with temperature decreasing treatment(TD)and temperature increasing treatment(TI)below 30℃,the HT about 30℃increased the RPP of R.esculentum 5.6 times and significantly improved the podocyst reproduction efficiency and the stocks number of R.esculentum’s polyps.We found that proper high temperature treatment can significantly improve the podocyst reproduction efficiency of R.esculentum.It is perspective to be an effective way to increase the stocks number amount of R.esculentum’s polyps.