摘要
游离酶价格昂贵,活性不稳定,在高温、强酸和强碱条件下易失活。纳米模拟酶性能稳定,成本低,具有类酶活性。通过同步还原法制备一种具有类过氧化氢酶活性的纳米模拟酶(Cu-rGO),并负载磁性纳米粒子,制备磁性纳米模拟酶(Cu-rGO-Fe_(3)O_(4)),进行谱学表征以及条件优化,最后用于H_(2)O_(2)的分解。
Free enzyme is expensive and unstable,and it is easy to be inactivated under high temperature,strong acid and strong alkali.Nano-mimetic enzyme has stable performance,low cost and enzyme-like activity.In this paper,a nano-mimetic enzyme(Cu-rGO)with catalase-like activity was prepared by synchronous reduction method,and magnetic nano-mimetic enzyme(Cu-rGO-Fe_(3)O_(4))was prepared by loading magnetic nanoparticles.The spectral characteristics and conditions optimization were investigated,and finally it was used for the decomposition of H_(2)O_(2).
作者
沈楠
华绍烽
Shen Nan;Hua Shaofeng(Henan Polytechnic University,Jiaozuo 454000,Henan,China)
出处
《工业催化》
CAS
2023年第10期42-47,共6页
Industrial Catalysis
基金
河南省教育厅科学技术重点研究项目(14B150026)
河南省科技厅科技攻关项目(142102210049)。
关键词
酶学
纳米模拟酶
磁性纳米粒子
过氧化氢酶
酶活性
enzymology
nanomimetic enzyme
magnetic nanoparticles
catalase
enzyme activity
