摘要
目的 通过血清非靶向代谢组学技术研究黄连解毒汤对健康SD大鼠生理功能的影响。方法 大鼠24只适应性喂养7 d后,随机分为空白组(N组)、黄连解毒汤高剂量组(H组)、中剂量组(M组)、低剂量组(L组),每组6只。N组灌胃生理盐水,H组、M组、L组分别按照每次6.250 g/kg、3.125 g/kg、1.560 g/kg剂量灌胃1 mL、每天2次、连续21 d。采取大鼠血清样本前禁食禁水12 h,经麻醉后腹主动脉取血并分离血清。通过GC-TOF-MS检测各组大鼠血清中代谢产物的变化,采用多元分析OPLS-DA以及单变量统计学方法筛选其差异代谢物,再经过MetaboAnalyst平台进行代谢通路分析。结果 与N组相比,H组、M组、L组大鼠血清中显著改变的代谢小分子主要有胆固醇、胆酸、核糖、白皮杉醇、皮质醇、油酸、琥珀酸、亚麻酸、醋酸和丙酮醇等(P<0.05)。相关分析表明黄连解毒汤能显著影响机体的初级胆汁酸代谢、丙氨酸、天冬氨酸和谷氨酸代谢、α-亚麻酸代谢、甘油磷脂代谢,且其发挥体内作用可能与摄入的剂量有关,为深入揭示黄连解毒汤促进胆汁酸代谢的作用机制提供一定参考。结论 黄连解毒汤能促进体内初级胆汁酸生成并增强胆汁酸代谢通路的作用。
Objective To study the effect of Huanglian Jiedu Decoction(HLJD)on the physiological function of healthy SD rats through serum non-targeted metabolomics.Methods After 7 days of adaptive feeding,24 rats were randomly divided into blank group(group N)and high-dose HLJD group(group H),medium-dose HLJD group(group M),and low-dose HLJD group(group L)with six rats per group.Group N was gavaged with physiological saline,group H,group M,and group L were gavaged with 6250 g/kg,3125 g/kg,and 1560 g/kg HLJD,respectively.Rats were gavaged with 1 mL twice per day for 21 d.Before collecting serum samples,fasting and water deprivation were performed for 12 h.After anesthesia,blood was collected from the abdominal aorta,and serum was separated.Changes in metabolic products in the serum were detected by Gas Chromatography Time-of-Flight Mass Spectrometry(GC-TOF-MS).Multivariate analysis by Orthogonal Projections to Latent Structures Discriminant Analysis(OPLS-DA)and univariate statistical method were used to screen differential metabolites,and then metabolic pathway analysis was conducted using the Metabo Analyst platform.Results Compared with the group N,metabolic small molecules were significantly altered in the serum of group H,group M,and group L,which mainly included cholesterol,cholic acid,ribose,paclitaxel,cortisol,oleic acid,succinic acid,linolenic acid,acetic acid,and acetone alcohol(P<005).Correlation analysis showed that HLJD significantly affected the metabolism of primary bile acids,alanine,aspartic acid,and glutamate inα-linolenic acid and glycerol phospholipid metabolism,and its in vivo effects may be related to the intake dose,providing a reference to further reveal the mechanism of HLJD in promoting bile acid metabolism.Conclusions HLJD promotes the production of primary bile acids in vivo and enhances the effects of the bile acid metabolic pathway.
作者
杨光勇
杜海洋
张庚鑫
苏钢
涂小华
何光志
YANG Guangyong;DU Haiyang;ZHANG Gengxin;SU Gang;TU Xiaohua;HE Guangzhi(Guizhou University of Traditional Chinese Medicine School of Basic Medicine,Guiyang 550025,China)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2023年第10期1278-1286,共9页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金地区科学基金项目(82060796)
贵州省科学技术基金项目(黔科合基础[2020]1Y392)
贵州省中医药管理局中医药民族医药科学技术研究课题(QZYY-2022-005)
贵州省科技创新人才团队建设专项(黔科合平台人才[2020]5010)
贵州省发展和改革委员会工程研究中心建设项目[黔发改高技(2020)896号]。
关键词
血清代谢组学
黄连解毒汤
气相色谱-飞行时间质谱
胆汁酸代谢
serum metabolomics
Huanglian Jiedu Decoction
GC-TOF-MS
bile acid metabolism Conflicts of Interest:The authors declare no conflict of interest.