驱动长刺蒺藜草成功入侵的根际土壤微生物群落研究

Study on Rhizosphere Soil Microbial Community Driving the Successful Invasion of Cenchrus longispinus

根际土壤微生物群落对外来入侵植物形成反馈是其成功入侵的重要原因之一。采用单因素完全随机试验设计,以入侵植物长刺蒺藜草根际土壤、本土植物狗尾草根际土壤及无植物种植土壤为对象,通过高通量测序技术研究三者的土壤微生物群落结构组成及其差异,并探讨其入侵机制。结果表明,与本土植物狗尾草根际土壤微生物群落相比,入侵植物长刺蒺藜草根际土壤细菌群落的多样性降低,根际土壤真菌群落的多样性提高;入侵植物长刺蒺藜草根际土壤主要细菌门为变形菌门(Proteobacteria)和放线菌门(Actinobacteria);LEfSe多级物种差异分析表明,蓝细菌(Cyanobacteria)和Methylomirabilota是长刺蒺藜草根际土壤的优势细菌。FAPROTAX功能预测结果显示,入侵植物长刺蒺藜草根际土壤细菌在光合营养作用、光能自养作用、氧性光能自养、蓝细菌等方面的功能类群均有较高的丰度。变形菌门(Proteobacteria)、鞘脂单胞菌属(Sphingomonas)、蓝细菌(Cyanobacteria)和Methylomirabilota是驱动长刺蒺藜草成功入侵的优势细菌。

The feedback of rhizosphere soil microbial community to alien invasive plants is one of the important reasons for their successful invasion.In order to explore the diversity of microbial community structure in the rhizosphere soil that drives the successful invasion of Cenchrus longispinus,a single-factor completely randomized experimental design was used to study the community structure and differences of rhizosphere soil microorganisms by high-throughput sequencing technology in the rhizosphere soil of the invasive plant Cenchrus longispinus(CP),native plant Setaria viridis(SV),and plantless soil(CK),and the invasion mechanism of Cenchrus longispinus was explored.The results showed that compared with the native plant Setaria viridis rhizosphere soil microbial community,the diversity of invasive plant Cenchrus longispinus rhizosphere soil bacterial community decreased,while the diversity of rhizosphere soil fungal community increased.The main bacterial phyla in the rhizosphere soil of the invasive plant Cenchrus longis⁃pinus were Proteobacteria and Actinobacteria.LEfSe(LDA effect size)multi-level species difference analysis showed that Cyanobacteria and Methylomirabilota were the dominant bacteria in the rhizosphere soil of Cenchrus longispinus.The function prediction results of FAPROTAX showed that the functional groups of bacteria in the rhizosphere soil of invasive plant Cenchrus longispinus were highly abundant in terms of photosynthesis nutrition,photoautotrophic activity,aerobic photoautotrophic activity,and cyanobacteria.Proteobacteria,Sphingomonas,Cyanobacteria,and Methylomirabilota were the dominant bacteria driving the successful invasion of Cenchrus longispinus.