‘粉色记忆’玉簪组培快繁技术初探

Study on Tissue Culture and Rapid Propagation Technology of Hosta plantaginea‘Tickle Me Pink’

[目的]研究‘粉色记忆’玉簪植物的离体快繁技术。[方法]以‘粉色记忆’玉簪叶片作为外植体进行快繁,研究不同灭菌方法、激素浓度配比对玉簪愈伤组织的诱导、增殖,芽增殖和生根的影响。[结果]外植体最佳消毒方法为用0.5%二氯异氰尿酸钠溶液消毒15 min,愈伤组织诱导最佳培养基为MS+1.0 mg/L TDZ+1.0 mg/L 2,4-D,愈伤组织增殖最佳培养基为MS+2.0 mg/L 6-BA+0.5 mg/L NAA,芽增殖最佳培养基为MS+1.0 mg/L TDZ+1.0 mg/L 2,4-D,生根最佳培养基为1/2 MS+0.1 mg/L NAA。[结论]该研究不仅可以丰富皖北地区的玉簪资源,满足市场需求,还可以提供玉簪组培实践依据和理论指导,为筛选优良无性系奠定基础。

[Objective]The aim was to study the in vitro rapid propagation technology of Hosta plantaginea‘Tickle Me Pink’.[Method]The leaves of H.plantaginea‘Tickle Me Pink’were used as the explants to study the effects of different sterilization methods,different kinds and concentrations of plant growth regulators on callus induction,callus proliferation,and plant regeneration.[Result]The optimal disinfection method for explants was to disinfect with 0.5%sodium dichloroisocyanurate solution for 15 minutes,the optimal culture medium for callus induction was MS+1.0 mg/L TDZ+1.0 mg/L 2,4-D,the optimal culture medium for callus proliferation was MS+2.0 mg/L 6-BA+0.5 mg/L NAA,the optimal culture medium for bud proliferation was MS+1.0 mg/L TDZ+1.0 mg/L 2,4-D,and the optimal culture medium for rooting was 1/2 MS+0.1 mg/L NAA.[Conclusion]This study can not only enrich the resources of H.plantaginea in northern Anhui and meet the market demand,but also provide practical basis and theoretical guidance for tissue culture of H.plantaginea,laying a foundation for screening excellent clones.