质谱成像技术前沿进展及其在药物研究中的应用

Recent advances in mass spectrometry imaging and its application in drug research

质谱成像(MSI)作为一种无标记的分子成像技术,弥补了传统液质联用等分析技术空间分辨能力的不足,已被广泛应用于小分子代谢物、脂质、多肽及蛋白质的组织分布研究。随着MSI技术灵敏度和空间分辨率的不断提高,该技术在精确定位药物组织分布、可视化药物代谢过程、追踪药物递送等研究领域备受关注,为药物临床前研究提供了新技术和新方法。本文介绍了多种常见MSI技术的基本原理、技术关键参数、技术优势与不足,重点综述了近年来MSI技术在药物有效性及安全性评价、药物组织分布研究、药物递送、中药分析等领域的应用,以期拓展MSI技术在药物研发中的应用,推动药物研发进程。

Mass spectrometry imaging(MSI),a label-free molecular imaging technique,has been applied widely in the spatial localization of small molecule metabolites,lipids,peptides,and proteins,with its unique advantage of high spatial resolving power compared to traditional liquid chromatography-mass spectrometry(LC-MS).With the nonstop advancement of its achievable sensitivity and spatial resolution,MSI technique has been providing novel perspectives into the preclinical studies of drugs,such as in vivo localization of drugs and their metabolites,visualization of drug metabolism,and drug delivery tracking.This review introduces the basics of MSI techniques,including basic principles,key features,technical advantages,and limitations,with particular highlight of the recent applications of MSI in drug efficacy and safety evaluation,drug distribution research,drug delivery research,and analysis of Chinese medicine from recent publications,aiming to promote the utilization and further expansion of MSI in the research and development of drugs.