维生素K_(2)对糖尿病性骨质疏松症大鼠骨组织和糖代谢指标的影响

Effects of vitamin K_(2) on bone tissue and glucose metabolism in diabetic osteoporosis rats

目的讨论维生素K_(2)对糖尿病性骨质疏松症(DOP)大鼠骨组织和糖代谢指标的影响。方法将18只12周龄雄性SD大鼠随机分为正常组、模型组和干预组,正常组不进行造模,模型组和干预组构建DOP模型。建模成功后,干预组给予维生素K_(2)灌胃,模型组和正常组均给予等量生理盐水灌胃。干预4周后分离并收集大鼠股骨,通过Micro-CT检测大鼠股骨远端骨密度(BMD)和骨形态相关参数;通过免疫组化染色观察大鼠破骨细胞数量;通过免疫荧光染色观察大鼠成骨细胞数量;通过力学检测仪观察大鼠骨组织力学性能;取大鼠尾部静脉血测定空腹胰岛素(FINS)、空腹血糖(FPG)水平;运用稳态模式评估法测定胰岛素抵抗指数(HOMA-IR)及胰岛素敏感指数(ISI)。结果干预组的BMD、骨体积分数、骨小梁数量、骨小梁厚度大于模型组(P<0.05)。免疫组化及免疫荧光染色结果显示,补充维生素K_(2)后破骨细胞数量显著减少,成骨细胞较模型组明显增加。干预组的极限载荷和刚度高于模型组(P<0.05)。干预组的FINS、FPG水平及HOMA-IR低于模型组,ISI高于模型组(P<0.05)。结论维生素K_(2)可显著减缓DOP大鼠的骨丢失量,改善糖代谢指标,为DOP的治疗提供了新思路。

Objective To investigate the effects of vitamin K_(2)on bone tissue and glucose metabolism in diabetic osteoporosis(DOP)rats.Methods Eighteen 12-week-old male SD rats were randomly divided into normal group,model group and intervention group.The normal group was not modeled,and the DOP model was constructed in the model group and the intervention group.After successful modeling,the intervention group was given vitamin K_(2)by gavage,and the model group and the normal group were given the same amount of normal saline by gavage.After 4 weeks of intervention,the femurs of rats were separated and collected,and the bone mineral density(BMD)and bone morphology related parameters of distal femur were detected by Micro-CT;the number of osteoclasts in rats was observed by immunohistochemical staining;the number of osteoblasts in rats was observed by immunofluorescence staining;the mechanical properties of bone tissue in rats were observed by mechanical detector;the levels of fasting insulin(FINS)and fasting plasma glucose(FPG)were measured in tail venous blood of rats;homeostasis model assessment of insulin resistance(HOMA-IR)and insulin sensitive index(ISI)were measured by homeostasis model assessment.Results The BMD,bone volume fraction,trabecular number and trabecular thickness of the intervention group were greater than those of the model group(P<0.05).The results of immunohistochemistry and immunofluorescence staining showed that the number of osteoclasts decreased significantly after vitamin K_(2)supplementation,and the number of osteoblasts increased significantly compared with the model group.The ultimate load and stiffness of the intervention group were higher than those of the model group(P<0.05).The FINS,FPG levels and HOMA-IR in the intervention group were lower than those in the model group,ISI was higher than that in the model group(P<0.05).Conclusion Vitamin K_(2)can significantly slow down the bone loss of DOP rats and improve the glucose metabolism indexes,which provides a new idea for the treatment of DOP.