猪繁殖与呼吸综合征对塞内卡病毒灭活苗免疫效果的影响

Effect of Porcine Reproductive and Respiratory Syndrome on Immunity of Inactivated Senecavirus A Vaccine

为探究猪繁殖与呼吸综合征(PRRS)对塞内卡病毒(SVA)灭活疫苗免疫效果的影响,对PRRS阳性猪和PRRS阴性猪分别免疫相同批次、相同剂量的SVA灭活苗,检测首次免疫后7 d、14 d、21 d、28 d血清中和抗体效价,并在免疫后28 d对所有试验猪进行攻毒保护试验。结果显示,所有PRRS阳性猪的SVA血清中和抗体效价均小于1∶4,而PRRS阴性猪SVA血清中和抗体效价在二免后上升到较高水平。攻毒保护结果与中和抗体效价结果呈正相关,PRRS阳性组0/5保护,而PRRS阴性组试验猪达到5/5保护。这一研究表明PRRS会使SVA灭活疫苗免疫效果下降,为SVA及PRRS疫苗的研发及猪场疾病的防控提供了参考。

In order to explore the effect of Porcine reproductive and respiratory syndrome(PRRS)on the immune effect of inactivated Senecavirus A(SVA)vaccine,the same batch and dose of inactivated SVA vaccine were immunized respectively in PRRS positive pigs and PRRS negative pigs in this experiment.Serum neutralizing antibody titers were detected at 7,14,21 and 28 days after the first immunization,and the challenge protection test was performed on all pigs at 28 days after immunization.The results showed that the titers of all serum neutralizing antibodies of the pigs with PRRS were less than 1∶4,while the titers of serum neutralizing antibodies of PRRS negative pigs rose to a high level after the enhanced immunization.The results of challenge protection were positively correlated with the titer of neutralizing antibody.The inactivated SVA vaccine provided 0/5 protection for the PRRSV positive group and 5/5 protection for the PRRS negative group.This study shows that PRRS can reduce the immune effect of inactivated SVA vaccine,which provides a reference for the development of SVA vaccine and PRRS vaccine and the prevention and control of swine diseases.