miR-146a及MUC5AC在慢性鼻-鼻窦炎伴鼻息肉组织中的表达及临床意义

The Expression and Clinical Significance of miR-146a and MUC5AC in Chronic Sinusitis with Nasal Polyps

目的:探讨微小RNA-146a(miR-146a)及黏蛋白5AC(MUC5AC)在慢性鼻-鼻窦炎伴鼻息肉组织中的表达及其临床意义。方法:选取2022年1月—2023年1月新疆医科大学第二附属医院耳鼻喉科所收治的慢性鼻-鼻窦炎伴鼻息肉患者40例(观察组)及同期因鼻外伤、鼻中隔偏曲、单纯鼻窦囊肿接受手术治疗患者40例(对照组)。检测对照组鼻黏膜组织和观察组鼻息肉组织的miR-146a水平及MUC5AC水平,并分析两组的VAS评分、鼻内镜评分,以及鼻息肉组织的miR-146a、MUC5AC表达水平与VAS评分、鼻内镜评分、炎症细胞百分比的相关性。结果:观察组miR-146a水平低于对照组,MUC5AC水平高于对照组,差异均有统计学意义(P<0.05);鼻息肉组织中的miR-146a水平与VAS评分及鼻内镜评分均呈负相关(r=-0.637、-0.339,P<0.05),而MUC5AC水平与VAS评分及鼻内镜评分均呈正相关(r=0.525、0.471,P<0.05);miR-146a的表达水平与嗜酸性粒细胞的百分比呈负相关(r=-0.701,P<0.05),MUC5AC的表达水平与嗜酸性粒细胞百分比呈正相关(r=0.587,P<0.05),miR-146a、MUC5AC的表达水平与中性粒细胞百分比、淋巴细胞百分比均无相关性(P>0.05)。结论:在慢性鼻-鼻窦炎伴鼻息肉疾病诊断中,鼻息肉组织的miR-146a水平低表达及MUC5AC水平高表达均提示病情加重,均与组织炎性程度有关。

Objective:To investigate the expression and clinical significance of microRNA-146a(miR-146a)and mucin 5AC(MUC5AC)in chronic sinusitis with nasal polyps.Method:From January 2022 to January 2023,40 patients with chronic rhinosinusitis with nasal polyps admitted to the Department of Otolaryngology,the Second Affiliated Hospital of Xinjiang Medical University(the observation group)and 40 patients with nasal trauma,deflection of the nasal septum,and simple sinus cyst received surgery at the same time(the control group)were selected.The miR-146a and MUC5AC levels in the control group nasal mucosal tissue and the observation group nasal polyp tissue were detected.The VAS score,nasal endoscopy score,as well as the correlation between miR-146a and MUC5AC expression levels in nasal polyp tissue and VAS score,nasal endoscopy score,and percentage of inflammatory cells in both groups were analyzed.Result:The level of miR-146a in the observation group was lower than that in the control group,and the level of MUC5AC was higher than that in the control group,the differences were statistically significant(P<0.05).The level of miR-146a in nasal polyps was negatively correlated with VAS score and nasal endoscopic score(r=-0.637,-0.339,P<0.05),while the level of MUC5AC was positively correlated with VAS score and nasal endoscopic score(r=0.525,0.471,P<0.05).The expression level of miR-146a was negatively correlated with the percentage of eosinophils(r=-0.701,P<0.05),and the expression level of MUC5AC was positively correlated with the percentage of eosinophils(r=0.587,P<0.05).The expression levels of miR-146a and MUC5AC were not correlated with the percentage of neutrophils and lymphocytes(P>0.05).Conclusion:In the diagnosis of chronic sinusitis with nasal polyp tissue diseases,the low expression of miR-146a and the high expression of MUC5AC levels indicate an aggravation of the condition and inflammatory degree.