嗅觉训练对3-甲基吲哚诱导的嗅觉障碍小鼠嗅觉功能的影响

Effect of olfactory training on olfactory function in mice with 3-methylindole-induced olfactory disorder

目的 探讨嗅觉训练对3-甲基吲哚诱导的嗅觉障碍小鼠嗅觉功能的影响。方法 选取8周龄、体重18~22 g的SPF级雄性C57BL/6J小鼠60只,按随机数字表法将其分为对照组、嗅觉障碍组、嗅觉训练组,每组20只。嗅觉障碍组、嗅觉训练组经3-甲基吲哚诱导建立嗅觉障碍模型,以小鼠200 s内未找到鼠料颗粒为嗅觉障碍模型建立成功。嗅觉训练组接受嗅觉训练,雾化吸入4种嗅素(玫瑰精油、柠檬精油、丁香精油、桉叶油醇),每种持续吸入30 min,2次/d,同时给予对照组、嗅觉障碍组等量蒸馏水雾化处理,三组均连续干预4周。比较三组觅食时间及嗅上皮功能的变化。结果 嗅觉障碍组建模后及训练1、2、4周后觅食时间均长于建模前及同期对照组,差异有统计学意义(P<0.05)。嗅觉训练组建模后及训练1、2、4周后觅食时间长于建模前,训练1、2、4、周后短于建模后,训练2、4周后短于训练1周后,训练4周后短于训练2周后,且嗅觉训练组训练1、2、4周后觅食时间短于同期嗅觉障碍组,差异有统计学意义(P<0.05)。训练4周后,嗅觉障碍组嗅觉标记蛋白阳性细胞数、嗅上皮细胞总数、嗅上皮厚度低于对照组,嗅觉训练组高于嗅觉障碍组,差异有统计学意义(P<0.05)。结论 嗅觉训练能够缩短嗅觉障碍小鼠觅食时间,改善嗅上皮功能,从而促进嗅觉功能恢复。

Objective To investigate the effect of olfactory training on the olfactory function of 3-methylindole induced olfactory dysfunction mice.Methods Sixty SPF grade male C57BL/6J mice aged eight weeks and weighing 18-22 g were selected and divided into control group,olfactory disorder group,and olfactory training group according to random number table method,with 20 mice in each group.The olfactory disorder model was established by 3-methylindole induction in the olfactory disorder group and the olfactory training group,and the model was successfully established with no rat granule found within 200 s.The olfactory training group received olfactory training,and inhaled four kinds of olfactory elements(rose essentialoil,lemonessentialoil,cloveessentialoil,andeolineol),eachofwhichwas continuouslyinhaled for 30 min,twice a day;meanwhile,the control group and the olfactory disorder group were given the same amount of distilled water atomization treatment,and the three groups were continuously interfered with for four weeks.Foraging time and olfactory epithelial function were compared among the three groups.Results The foraging time after setting up the model and after 1,2,4 weeks of training in olfactory disorder group was longer than that before modeling and in the same period of control group,the differences were statistically significant(P<0.05).The foraging time of olfactory training group after setting up the model and after 1,2,4 weeks of training was longer than that before modeling;the foraging time after 1,2,4 weeks of training was shorter than that after modeling,the foraging time after 2,4 weeks of training was shorter than that after training one week,and the foraging time after training four weeks was shorter than that after training two weeks,and the foraging time after 1,2,4 weeks of training was shorter than that in the same period of olfactory impairment group,the differences were statistically significant(P<0.05).After four weeks of training,the number of olfactory marker protein positive cells,the total number of olfactory epithelial cells,and the thickness of olfactory epithelial cells in the olfactory disorder group were lower than those in the control group,and those of olfactory training group were higher than those of olfactory disorder group,and the differences were statistically significant(P<0.05).Conclusion Olfactory training can shorten the foraging time of mice with olfactory impairment,improve the function of olfactory epithelium,and promote the recovery of olfactory function.