基于含水量-电导率拟合曲线模型优化丹参酮提取物微乳的研究

Optimization Based on Water Content Conductivity Fitting Curve Model Study on Tanshinone Microemulsion

目的 证实“含水量-电导率拟合曲线”模型测定微乳水包油(O/W)型相临界点的精确性,进一步使用该模型优化丹参酮提取物微乳。方法 以“含水量-电导率拟合曲线”模型确定微乳转变为O/W型的相临界点,根据微乳油相、总表面活性剂、水相占比,使用Origin 2021软件绘制伪三元相图,筛选成乳区域大的各相配比;以微乳的平均粒径、多分散系数(PDI)为考察指标,星点设计-响应面法优化微乳;采用高速离心和4、25℃下静置60 d,考察微乳的稳定性。结果 优化后的微乳处方为(含药油相为18.11%,聚山梨酯80为61.21%,1,2-丙二醇为20.68%,临界点含水量为64.29%),Km为2.96,配制温度为27.4℃,不同实验条件下微乳的稳定性均良好。结论 “含水量-电导率拟合曲线”模型确定的O/W型相临界点精确性高,优化后的微乳稳定性好,达到了预期的研究目标。

OBJECTIVE To verify the accuracy of the“water content-conductivity fitting curve”model for determining the critical point of microemulsion O/W phase,and to further use this model to optimize the microemulsion of tanshinone extract.METHODS The“water content-conductivity fitting curve”model was used to determine the phase critical point of microemulsion into O/W type.According to the proportion of microemulsion oil phase,total surfactant and water phase,the pseudo ternary was drawn using Origin 2021 software.The phase diagram was used to screen out the proportions of the larger milk-forming areas;the average particle size and PDI of the microemulsion were used as the inspection indicators,and the star point design-response surface method was used to optimize the microemulsion;high-speed centrifugation and standing at 4℃and 25℃for 60 days,to examine the stability of the microemulsion.RESULTS The optimized microemulsion formulation was(18.11%medicated oil phase,61.21%Tween 80,20.68%1,2-propanediol,the water critical point is 64.29%),Km was 2.96,preparation temperature was 27.4℃,under different experimental conditions,the stability of the microemulsion was good.CONCLUSION The O/W phase critical point determined by the“water content-conductivity fitting curve”model has high accuracy,and the optimized microemulsion has good stability,which achieves the expected research goals.