1800 MHz电磁辐射暴露对大鼠海马GFAP表达的“窗口效应”

The“Window Effect”Study on the Effect of 1800 MHz Electromagnetic Radiation Irradiation on GFAP Expression in the Hippocampus of Rats

目的在1800 MHz电磁波照射下,研究电磁波功率密度对SD大鼠海马胶原纤维酸性蛋白(GFAP)表达的影响,以及是否具有“窗口效应”。方法将98只4周龄SPF级SD大鼠随机分为14个组,每组7只,7组暴露组(频率:1800 MHz,功率密度:0.1 mW/cm^(2)、0.3 mW/cm^(2)、0.5 mW/cm^(2)、0.7 mW/cm^(2)、0.9 mW/cm^(2)、1.0mW/cm^(2)、1.2 mW/cm^(2))和7组对照组(功率密度:0 mW/cm^(2)),每天暴露12 h,持续3周。暴露结束后,采用Western Blot检测海马组织的GFAP表达水平,免疫组化法测定海马组织中DG、CA3和CA1区域的GFAP阳性表达产物平均光密度(MOD)值,以确定在1800 MHz暴露下的SD大鼠海马中GFAP表达的功率密度窗口。结果在0.1 mW/cm^(2)和0.3 mW/cm^(2)功率密度下,Western Blot结果表示,可增加大鼠海马GFAP表达量(P<0.05)以及免疫组化染色显示可增加3个区域GFAP的MOD值(P<0.05)。结论长时间暴露于1800 MHz电磁辐射对SD大鼠海马DG区、CA3区和CA1区的GFAP表达有影响具有“窗口效应”,强度窗口的功率密度为0.1 mW/cm^(2)和0.3 mW/cm^(2)。

Objective To investigate the effect of electromagnetic wave power density on the expression of glial fibrillary acidic protein(GFAP)in the hippocampus of SD rats under 1800 MHz electromagnetic wave irradiation,and whether it exhibits a“window effect”.Methods Ninety-eight 4-week-old SPF-grade SD rats were randomly divided into 14 groups,with 7 rats in each group.Seven groups were exposed groups(frequency:1800 MHz,power densities:0.1 mW/cm^(2),0.3 mW/cm^(2),0.5 mW/cm^(2),0.7 mW/cm^(2),0.9 mW/cm^(2),1.0 mW/cm^(2),1.2 mW/cm^(2))and corresponding 7 groups were control groups(power density:0 mW/cm^(2)).Exposure was conducted for 12 hours daily for 3 weeks.After exposure,Western Blot was used to detect the expression level of GFAP in the hippocampal tissue,and immunohistochemistry staining was performed to determine the average optical density(MOD)value of GFAP-positive expression products in the DG,CA3,and CA1 regions of the hippocampal tissue,to determine the power density window of GFAP expression in the hippocampus of SD rats under 1800 MHz exposure.Results At power densities of 0.1 mW/cm^(2)and 0.3 mW/cm^(2),Western Blot results showed increased expression of GFAP in the rat hippocampus(P<0.05),and immunohistochemistry staining demonstrated increased MOD values of GFAP in the three regions(P<0.05).Conclusion Long-term exposure to 1800 MHz electromagnetic radiation has a“window effect”on the expression of GFAP in the DG,CA3,and CA1 regions of the hippocampus in SD rats,with power density windows of 0.1 mW/cm^(2)and 0.3 mW/cm^(2).