牛膝多糖对雏鸡免疫功能的影响

Effect of achyranthes bidentata polysaccharides on immune function of chicks

文章旨在研究牛膝多糖对雏鸡免疫功能的影响。试验将120只1日龄雏鸡随机分为4组,每组3个重复,每个重复10只鸡(公母各半)。在常规饲料中分别添加200、150、100、0 mg/kg牛膝多糖。所用雏鸡连续饲喂至30日龄,每组取10只进行取样。计算动物机体免疫器官指数,MTT检测T细胞增殖活性,Griess检测巨噬细胞释放NO能力,ELISA检测血清总IgG水平。结果表明,在雏鸡饲料中添加中剂量(150 mg/kg)的牛膝多糖能显著提高雏鸡免疫器官指数、T细胞增殖活性、巨噬细胞释放NO能力和血清总IgG水平(P<0.05)。结论:牛膝多糖在一定程度上对雏鸡的免疫功能具有增强作用,从而提高机体抗病能力,饲料中牛膝多糖最适添加量为150 mg/kg。

To study the effect of achyranthes bidentata polysaccharides on immune function of chicks.Randomly divide 1201-day-old chicks into 4 groups,with 3 replicates in each group,with 10 chicks per replicate(half male and half female).200 mg/kg,150 mg/kg,100 mg/kg and 0 mg/kg achyranthes bidentata polysaccharides were added to the conventional feed.The chicks were fed continuously until the age of 30 days,and 10 chicks from each group were sampled.The immune organ index of animals was calculated,the proliferative activity of T cells was detected by MTT,the ability of macrophages to release NO was detected by Griess,and the serum total IgG level was detected by ELISA.The results showed that,Adding medium dose(150 mg/kg)of Achyranthes bidentata polysaccharide to chicken feed can significantly improve the immune organ index,T cell proliferation activity,NO release ability of macrophages and total serum IgG level of chicks.Conclusion:Achyranthes bidentata polysaccharides can enhance the immune function of chicks to a certain extent,thus improving the disease resistance of the body.The dosage of Achyranthes bidentata polysaccharides added in the feed is 150 mg/kg.