不同氧气含量条件下无水保活珍珠龙胆石斑鱼品质预测模型的建立

Establishment of Quality Prediction Model of Pearl Gentian Grouper During Waterless Keeping Alive with Different Oxygen Content

为探究氧气含量对无水保活珍珠龙胆石斑鱼品质的影响,分析了包装内不同氧气体积分数(60%、80%、99%)时珍珠龙胆石斑鱼低温无水保活前、保活期间(0、3、6、9、12 h)和复水12 h后生理指标和肌肉品质的变化,并建立了品质预测模型。结果表明,当氧气体积分数为99%时,珍珠龙胆石斑鱼血浆葡萄糖和皮质醇质量浓度随保活时间的延长显著增加(P<0.05),最大值分别为(0.86±0.01)mg/mL和(3034.21±14.84)ng/L,肌肉弹性由0.79±0.07下降到0.50±0.01,黏聚性由0.61±0.01下降到0.51±0.00。当氧气体积分数为60%时,石斑鱼血浆谷丙转氨酶、谷草转氨酶活性和肌酐质量浓度在保活期间处于稳定上升的趋势,肌肉糖原质量比由(1.47±0.02)mg/g下降到(0.71±0.02)mg/g,从而积累了大量的乳酸。相比之下,氧气体积分数为80%时的石斑鱼处于相对较低的应激水平,因此,可以更积极地适应低温无水环境。建立了以血浆葡萄糖、肌肉糖原、弹性、黏聚性为石斑鱼健康品质关键指标的预测模型,该模型拟合程度良好,可为石斑鱼实际运输过程中健康品质指标的挖掘提供参考。

To investigate the effect of oxygen content on the quality of pearl gentian grouper during waterless keeping alive,the changes of physiological indicators and muscle quality of pearl gentian grouper before waterless keeping alive,during keeping alive(0,3,6,9,12 h)and after revival for 12 h under different oxygen volume fraction(60%,80%,99%)in package were analyzed,and the quality prediction model was established.The results showed that when the oxygen volume fraction was 99%,the plasma glucose and cortisol mass concentrations of pearl gentian grouper were significantly increased with the prolongation of keeping alive time(P<0.05),and the maximum values were(0.86±0.01)mg/mL and(3034.21±14.84)ng/L,respectively.In addition,muscle springiness decreased from 0.79±0.07 to 0.50±0.01,and cohesiveness declined from 0.61±0.01 to 0.51±0.00.When the oxygen volume fraction was 60%,the activities of alanine aminotransferase,aspartate aminotransferase and the mass concentration of creatinine in plasma were increased steadily during the keeping alive period.The mass ratio of glycogen in muscle decreased from(1.47±0.02)mg/g to(0.71±0.02)mg/g,resulting in the accumulation of a large amount of lactic acid.In contrast,grouper under 80%oxygen volume fraction could more actively adapt to low temperature and anhydrous environment because of a relatively low stress level.Finally,the quality prediction model was established with plasma glucose,muscle glycogen,springiness and cohesiveness as key quality indexes for the health quality of grouper,and fitting degree of which was good.This study could provide reference for the excavation of health quality indicators during the actual transportation process of grouper.