浦东新区HIV-1不同亚型感染者的肠道菌群特征及其与血脂异常的关联

Characterization of intestinal flora in various genetic subtypes of HIV-infected patients and its association with dyslipidemia in Pudong New Area,China

目的了解浦东新区不同基因亚型的HIV感染者肠道菌群的差异及其与血脂指标之间的关系。方法收集HIV感染者(HIV+组)和年龄、BMI相匹配的HIV阴性人群(HIV-组)的粪便样本,进行16SrDNA测序,并对HIV+组进行基因亚型测定,同时测定外周血血脂水平并进行生物信息学分析,采用Spearman秩相关分析肠道菌群-血脂指标相关性。结果纳入HIV+组和HIV-组各47例,均为男性,其中HIV感染者基因亚型包括CRF01_AE组(25例)和CRF07_BC组(22例)。CRF01_AE组的血脂指标HDL-C异常情况高于CRF07_BC组(P<0.05)。HIV+组和HIV-组的β多样性差异有统计学意义(R^(2)=0.0346,P<0.05)。在CRF07_BC组中观察到来自厚壁菌门的5种肠道细菌富集(LDA均>2.0),包括gauvreauii瘤胃球菌属、Dorea属、颤杆菌克属、CAG_56菌属、乳球菌属(P均<0.05)。HIV感染者的HDL-C水平和Dorea菌呈正相关(r=0.302,P=0.039)。结论不同基因亚型的HIV感染者的肠道菌群特征存在差异,HDL-C异常情况与差异菌种存在相关性,还需进一步的研究。

Objective To investigate the differences in intestinal flora among HIV-infected patients with various genotypes and their correlation with blood lipid indices in Pudong New Area.Methods Stool samples were collected from HIV-infected patients(HIV+group)and age-BMI-matched HIV-negative individuals(HIV-group)for 16S rDNA sequencing,and the genetic subtype were tested in HIV+group.Additionally,lipid levels in peripheral blood were quantitatively determined and subsequently analyzed bioinformatically.Spearman's rank order correlation was employed to analyze the correlations between intestinal flora and lipid indices.Results A total of 47 HIV-infected patients and 47 HIV-negative individuals were included,all of whom were men.The HIV-infected genetic subtypes comprised the CRF01_AE group(25 patients)and the CRF07_BC group(22 patients).The CRF01_AE group exhibited higher abnormalities in the lipid index,specifically high-density lipoprotein cholesterol(HDL-C),than the CRF07_BC group(P<0.05).Statistical differences inβdiversity were observed between HIV+group and HIV-group(R^(2)=0.0346,P<0.05).Enrichment of five intestinal bacteria from the phylum Firmicutes was noted in the CRF07_BC group(LDA all>2.0).These included Ruminococcus gauvreauii,Dorea,Oribacterium,CAG_56,and Lactococcus(P all<0.05).A positive correlation was identified between Dorea and HDL-C levels in HIV-infected patients(r=0.302,P=0.039).Conclusions There are differences in the gut flora characteristics of HIV-infected patients with different genetic subtypes and a correlation between HDL-C abnormalities and differential strains exists,necessitating further study.