脂肪酸对牛樟芝液态发酵产三萜的影响

Effect of fatty acids on triterpenoid production by liquid fermentation of Antrodia camphorata

为提高牛樟芝菌丝体产三萜类化合物的能力,以5种常见的脂肪酸为外源添加物,筛选出促进牛樟芝菌丝体生长和提高总三萜得率的最优培养基及培养方法,并对比分析空白对照组和添加脂肪酸的实验组所得菌丝体形态、发酵液颜色及黏度的差异。研究结果表明:不同脂肪酸的添加对牛樟芝菌丝体产三萜均有促进作用,其中亚麻酸对三萜生产的促进效果最强,其他脂肪酸的促进效果排序为亚油酸>油酸>硬脂酸>软脂酸;在添加亚麻酸的最优条件下(添加2.0 g/L亚麻酸、培养8 d)获得最大三萜得率为(5.0±0.1)%,是空白对照组的2.1倍。该研究方法操作容易,成本较低,效果显著,是促进牛樟芝菌丝体生长和三萜合成的有效策略,为牛樟芝三萜的规模化发酵提供参考和借鉴。

To enhance the triterpenoids production ability of Antrodia camphorata mycelium,five common fatty acids were used as exogenous additives to determine the optimal medium and culture method.Additionally,differences of mycelial morphology,fermentation fluid color,and viscosity between the blank control group and the experimental group supplemented with fatty acids were compared and analyzed.The results showed that different fatty acids could promote the production of triterpenoids.Among them,linolenic acid exhibited the strongest triterpenoid promoting effect,while the promoting effect of other fatty acids followed a decreasing order of linoleic acid>oleic acid>stearic acid>palmitic acid.Under the optimal conditions of adding linolenic acid(2.0 g/L linolenic acid added,culturing for 8 days),the maximum yield of total triterpenoids was(5.0±0.1)%,which was 2.1 times of that of the blank control group.The method employed in this study was easy to operate,cost-effiective,and efficienct.It represents an effective strategy for promoting the growth of mycelium and triterpenoids synthesis of Antrodia camphorata,providing a significant reference for the large-scale fermentation of Antrodia camphorata triterpenoids.