红曲菌利用甘油产色素及莫纳可林K的动态过程探究

Study on the dynamic process of pigments and Monacolin K production in Monascus by glycerol

红曲色素(Monascus pigments,MPs)和莫纳可林K(Monacolin K,MK)是红曲菌(Monascus spp.)的主要次级代谢产物。有研究表明,甘油可促进红曲菌产MPs与MK,但作用机制不明确。采用分光光度法、液相色谱法及气相色谱法等分析甘油对红曲菌生长、产MPs及MK和甘油利用等的影响。结果表明,与对照相比,添加甘油后红曲菌发酵液的pH降低、生物量增加,红曲菌产MPs及MK的量均得到提升(p<0.05)。当培养基中甘油浓度分别为40 g/L和160 g/L时,甘油的利用率随着发酵时间的延长而提升,分别在12 d和15 d时甘油利用率达到最高。发酵过程中,甘油主要分布在胞外。随着发酵时间的推移,甘油在胞内的比率有所提升。该结果为利用甘油促进红曲菌产MPs及MK提供一定的理论和技术支撑。

Monascus pigments(MPs)and monacolink(MK)are the main secondary metabolites of Monascus spp.Studies found that the productions of MPs and MK in Monascus spp.could be promoted by glycerol,but its function mechanism was not clear.In this study,analyzed the effect of glycerol on the growth,MPs and MK production,glycerol utilization of Monascus spp.by using spectrophotometry,liquid chromatography and gas chromatography,etc.The results showed that the pH of fermentation broth decreased while the biomass increased after adding glycerol.In addition,MPs and MK production were promoted significantly(p<0.05).Moreover,when the glycerol concentration of the culture medium were 40 g/L and 160 g/L,the glycerol utilization rate increased with the fermentation time,and the utilization rate came to the highestat 12 d and 15 d.During fermentation process,glycerol mainly distributed outside the cell.The rate of glycerol in cell increased along with the fermentation time.These findings will provide theoretical reference and technical support for using glycerol to promote MPs and MK production in Monascus spp.