HS-GC-MS结合代谢组学分析不同产地山药的挥发性成分

Analysis of Volatile Components of Chinese Yam from Different Origins by HS-GC-MS Combined with Metabolomics

目的:分析山药挥发性成分的组成及代谢功能,并探讨其挥发性成分与活性成分积累的相关性。方法:采集不同产地成熟期山药及对应产地饮片,利用顶空气相色谱质谱联用法(HS-GC-MS)进行挥发性成分的鉴定,利用京都基因与基因组百科全书通路(KEGG)结合代谢物的相关性及聚类分析,对挥发性成分进行非靶向代谢组学分析,鉴定不同产地山药的挥发性成分的差异及代谢功能。结果:不同产地山药的挥发性成分组成存在较大差异,河南焦作和河北蠡县山药挥发性成分的组成和相对百分含量变化趋势类似,且高于湖北武穴山药;不同产地的新鲜山药中醛类、醇类、酸类、烯类物质,以及共有组分呋喃-2-甲醛、5-甲基呋喃-2-甲醛及三甲基黄嘌呤的相对百分含量均较高;丙酸酯代谢通路富集丰富度最显著,其次是糠醛降解代谢和三甲基黄嘌呤代谢通路,这些代谢通路关系到生物机体碳水化合物代谢紊乱相关疾病。结论:山药的挥发性成分组成与产地、炮制方法相关联,挥发性成分的代谢组学分析以及功能分析为山药的活性物质基础研究提供了新的思路。

Objective:To reveal the composition and metabolic functions of volatile components of yam,and to explore the correlation between volatile components and the accumulation of active ingredients in yam.Methods:The mature yam and the corresponding origin tablets were collected from different origins.HS-GC-MS was used for the identification of volatile components.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway annotation,combined with correlation and clustering analysis of metabolites,were performed to analyze the non-targeted metabolomics analysis of volatile components to elucidate the main composition and metabolic functions of volatile components.Results:The composition of volatile components of yams from different origins all varied greatly,and the composition and relative percentages of volatile components of yams from Jiaozuo in Henan provine and Lixian in Hebei province showed similar trends and were higher than those from Wuxian in Hubei province.The relative percentages of aldehydes,alcohols,acids,alkenes,the common components of furan-2-carboxaldehyde,5-methylfuran-2-carboxaldehyde and trimethylxanthine were higher in fresh yams of different origins and were the main metabolic function substances annotated by KEGG metabolic pathway enrichment analysis.The propionate metabolic pathway was the most significantly enriched,and second were the matabolic pathway of glycolaldehyde degradation and thrimethylxanthine,which were related to the diseases of disorders of carbohydrate metabolism in living organisms.Conclusion:The composition of volatile components of yam is closely related to the origin and preparation method.The metabolomic analysis and functional analysis of volatile components provide a new idea for the basic research of active substances of Chinese yam.