微米尺度的规则拓扑结构对细胞和组织行为的影响

Impact of micro-scale regular topography on cell and tissue behaviors

生物材料表面的物理性质,如拓扑结构,由于其易于加工和稳定的生物作用而受到广泛关注.虽然已有许多研究报道了材料表面拓扑结构与细胞的相互作用,但由于不同研究中使用的微结构和实验环境的多样性,生物材料表面不同微结构与细胞/组织行为之间的影响关系尚未得到很好的统一评价.在本研究中,我们设计了11种图案(三角形、矩形、圆形等),这些图案包含两种类型的形貌(微坑和微柱),这样一共得到22种表面拓扑结构.我们在体外和体内对这些微结构影响细胞和组织的生物作用进行了评估.我们的研究结果表明,由连续微坑形成的凹槽微结构促进细胞迁移效果相比其他微结构更强.其中,由三角形微坑组成的凹槽在体外对细胞迁移的促进作用最强.在大鼠皮下模型中,这种三角形微坑组成的凹槽结构减少了纤维组织的包封,促进了血管的形成.

Physical cues of a biomaterial surface such as topography have received extensive attention due to their ease of engineering and reproducible bioeffects.Although many studies have been reported,the relationship between the topography of biomaterial surface and cell/tissue behaviors could not be well evaluated due to the diversified topographies and experimental environments used in these studies.Here we designed 11 patterns(triangle,rectangle,circle,etc.),and employed them to fabricate two types of topographies(micropit and microcolumn),resulting in 22 surface topographies.We conducted comparative assessments of the bioeffects of these microtopographies on cell and tissue in vitro and in vivo.Our results revealed that grooves formed by continuous micropits exhibited a greater ability to accelerate cell migration.Among them,grooves composed of triangular micropits exhibited the strongest enhancement of cell migration in vitro,reducing fibrous tissue encapsulation and promoting vascular formation in vivo.