食用肉类中5种动物源性成分多重PCR检测方法及检测试剂盒的研制

Development of a Multiplex PCR Detection Method and Detection Kit for Five Animal Derived Components in Edible Meat

目的:建立一种同步检测肉类中鹿、牛、羊、猪、马动物源性成分的多重聚合酶链式反应检测方法,并研发快速检测的多重PCR试剂盒。方法:将牛、羊、猪、马的Cyt b基因以及梅花鹿、马鹿的COX 1基因作为设计引物的靶序列,设计特异性引物,优化PCR体系,建立5种动物源性成分的多重PCR鉴定方法。结果:在同一反应体系中,可同步扩增鹿肉(173 bp)、牛肉(148 bp)、猪肉(261 bp)、羊肉(100 bp)以及马肉(424 bp)的特异性条带。结论:本研究开发的多重PCR检测试剂盒可同步检测肉类中5种动物源性成分。

Objective:To establish a multiplex polymerase chain reaction detection method for synchronous detection of animal derived components from deer,cattle,sheep,pigs,and horses in meat and develop a rapid detection multiplex PCR kit.Method:Based on the Cyt b genes of cattle,sheep,pigs,and horses,as well as the mitochondrial COX 1 genes of sika deer and red deer,as target gene sequences for primer design,five specific primers were designed,and the PCR system was optimized to establish a multiplex PCR identification method for five animal derived components.Result:In the same reaction system,a specific band of deer meat(173 bp),beef(148 bp),pork(261 bp),lamb(100 bp),and horse meat(424 bp)can be simultaneously amplified.Conclusion:The multiplex PCR detection kit in this study can simultaneously detect five animal derived components in meat.