血清HSP70、CCR3水平对细菌性肺炎患儿病情评估的价值

Value of serum HSP70,CCR3 for disease assessment in children with bacterial pneumonia

目的 探讨血清热休克蛋白70(HSP70)、CC趋化因子受体3(CCR3)表达水平对细菌性肺炎患儿病情评估的临床价值。方法 选取2022年6月至2023年6月湖北医药学院附属国药东风总医院就诊的细菌性肺炎患儿113例作为研究对象,按照病情严重程度分为轻症组71例和重症组42例。收集2组患儿的性别、年龄、体重指数,并测定肺功能[呼吸频率(RR)、潮气量(VT)、达峰容积比(VPTEF/VE)]等临床资料。采集清晨空腹外周静脉血5 mL,采用酶联免疫吸附试验法检测血清HSP70、CCR3水平,利用受试者工作特征(ROC)曲线分析血清HSP70、CCR3水平对细菌性肺炎患儿的病情评估的预测价值;采用Pearson分析血清HSP70、CCR3水平与病情严重程度的关系。结果 2组患儿在性别、年龄、体重指数等一般资料比较,差异无统计学意义(P>0.05);重症组血清HSP70、CCR3与RR明显高于轻症组,VT、VPTEF/VE显著低于轻症组,差异均有统计学意义(P<0.05)。血清HSP70联合CCR3评估细菌性肺炎患儿的AUC为0.916,明显高于单独检测(Z=2.480,3.322,P<0.05)。细菌性肺炎患儿病情严重程度与血清HSP70、CCR3表达水平呈正相关(r=0.826,0.778,P<0.05)。结论 细菌性肺炎患儿血清HSP70、CCR3表达水平明显升高,与病情严重程度密切相关,可作为细菌性肺炎患儿病情严重评估的临床指标。

Objective To explore the value of expression level of serum heat shock protein 70(HSP70) and CC chemokine receptor 3(CCR3) for disease assessment in children with bacterial pneumonia(BP).Methods A total of 113 children with BP who were admitted to the Sinopharm Dongfeng General Hospital,Hubei University of Medicine from June 2022 to June 2023 were recruited,they were assigned to the mild illness group(n=71) and the severe illness group(n=42) according to the condition.The gender,age,body mass index(BMI),and clinical data such as lung function index(respiratory rate[RR],tital volume[VT],the ratio of volume to peak expiratory flow to total expiratory volume[VPTEF/VE]) were collected between groups.Enzyme-linked immunosorbent assay was used to detect serum levels of HSP70 and CCR3 of collecting 5mL of fasting venous blood in the morning.Receiver operating characteristic(ROC) curve was plotted to analyze the predictive value of serum levels of HSP70 and CCR3 for disease assessment in BP children.Pearson analysis was used to investigate the relationship between serum levels of HSP70 and CCR3 with the severity of the condition.Results There was no statistically significant difference in general data such as gender,age,and BMI between the two groups(P>0.05).The serum levels of HSP70,CCR3,and RR in the severe group were significantly higher than those of the mild group,while VT and VPTEF/VE were significantly lower(P<0.05).The areas under curve(AUC) of the combination of serum HSP70 and CCR3 for BP children was 0.916,significantly higher than that single measured(Z=2.480,3.322,P<0.05).The severity of the condition in BP children was significantly positively correlated with the expression levels of serum HSP70 and CCR3(r=0.826,0.778,P<0.05).Conclusion The expression levels of serum HSP70 and CCR3 in BP children are significantly increased,which is closely related to the severity of the disease.It can be used as a clinical indicator for evaluating the severity of disease in BP children.