益生菌发酵提取榆耳抗氧化肽工艺优化及其抗运动疲劳作用研究

Study on optimization of extraction process of Gloeostereum incarnatum antioxidant peptide by probiotic fermentation and its anti-exercise fatigue effects

以榆耳为原料,采用益生菌发酵提取榆耳抗氧化肽。以ABTS自由基清除率为指标,筛选益生菌并优化沉淀pH,再在单因素试验基础上,采用响应面法优化益生菌发酵提取榆耳抗氧化肽的工艺,并研究榆耳抗氧化肽对运动大鼠的抗疲劳作用。结果表明:最优发酵工艺条件为使用枯草芽孢杆菌、沉淀pH为6液料比4.0∶1(mL/g)、发酵温度34℃、榆耳用量53 g、发酵时间24 h。在此条件下,榆耳抗氧化肽对ABTS自由基的清除率为93.44%。抗氧化能力试验表明,分子质量小于3 kDa的榆耳抗氧化肽的抗氧化活性最好。运动大鼠抗疲劳试验表明,灌胃榆耳抗氧化肽可以提高大鼠的耐力和抗疲劳能力。

Using Gloeostereum incarnatum as raw material,the antioxidant peptide was extracted by probiotic fermentation.Using ABTS free radical scavenging rate as an indicator,probiotic was screened and precipitation was optinized.On the basis of single factor experiments,response surface methodology was used to optimize the process of extracting antioxidant peptides from Gloeostereum incarnatum by probiotic fermentation,and the anti fatigue effect of Gloeostereum incarnatum antioxidant peptides on exercise rats were studied.The results showed that the optimal fermentation conditions for preparing Gloeostereum incarnatum antioxidant peptides were Bacillus subtilis as probiotics,precipitation pH 6,liquid to material ratio 4.0:1(mL/g),fermentation temperature 34℃,dosage of Gloeostereum incarnatum 53 g,fermentation time 24 h.Under these conditions,scavenging rate of Gloeostereum incarnatum antioxidant peptides on the ABTS free radical was 93.44%.The antioxidant capacity test showed that the activity of Gloeostereum incarnatum antioxidant peptides was best when its molecular weight was less than 3 kDa.The antifatigue test of exercise rats showed that gavage of Gloeostereum incarnatum antioxidant peptides could improve endurance and anti-fatigue ability of rats.