LncRNA LINC01133在侵袭性牙周炎中的表达及作用

Expression and role of lncRNA LINC01133 in invasive periodontitis

目的:探讨lncRNA LINC01133在侵袭性牙周炎中的作用及机制。方法:用qRT-PCR检测不同正常人群及侵袭性牙周炎患者中不同炎症状态下牙龈组织中LINC01133、基质金属蛋白酶9(MMP-9)的表达,并用ELISA法检测其白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)炎症细胞因子。构建侵袭性牙周炎细胞模型,通过大肠杆菌脂多糖刺激人牙龈成纤维细胞(HGFs),体外培养检测HGFs细胞中LINC01133表达水平的变化。构建LINC01133过表达载体,转染HGFs,用qRT-PCR和免疫印迹检测载体相关mRNA及蛋白表达水平的变化。结果:侵袭性牙龈炎患者牙龈组织中LINC01133呈现低表达,MMP-9、IL-6、TNF-α呈现高表达,且LINC01133、MMP-9两者呈负相关。经过炎症细胞模型构建,HGFsLINC01133呈现低表达,且经过转染后上调LINC01133的表达,可以抑制MMP-9、IL-6、TNF-α的表达,从而减轻炎症的发生,改善侵袭性牙周炎的进展。结论:炎性牙龈组织中的LINC0113低表达,提示其参与侵袭性牙周炎的发病过程,且LINC01133可调控MMP-9、IL-6、TNF-α的表达,以调控侵袭性牙周炎的变化。

Objective:To explore the role and mechanism of lncRNA LINC01133 in invasive periodontitis.Methods:The expressions of LINC01133 and matrix metalloproteinase-9(MMP-9)in gingival tissues of different groups of healthy population and invasive periodontitis patients under different inflammatory conditions were detected by qRT-PCR,and IL-6 and TNF-αinflammatory cytokines were detected by ELISA.The invasive periodontitis cell model was established and human gingival fibroblasts(HGFs)were stimulated by lipopolysaccharide of Escherichia coli.The expression of LINC01133 in HGFs cells was detected in vitro.The LINC01133 overexpression vector was constructed to transfect HGFs,and the changes of mRNA and protein secretion of the above factors in the HGFs were detected by qRT-PCR and Western blotting.Results:Low expression of LINC01133 and high expression of MMP-9,IL-6,and TNF-αwere found in human gingival tissues.There was an inverse correlation between LINC01133 and MMP-9.After the construction of inflammatory cell model,it was found that LINC01133 showed a low expression.Furthermore,after transfection the expression of LINC01133 was upregulated in the HGFs,and it was found to inhibit the expression of MMP-9,IL-6 and TNF-α,thus reducing the occurrence of inflammation and improving the progression of invasive periodontitis.Conclusions:The low expression of LINC0113 in inflammatory gingival tissues suggests its involvement in the pathogenesis of invasive periodontitis.Moreover,LINC01133 can regulate the expression of MMP-9,IL-6,and TNF-αto control the progression of invasive periodontitis.