不同木豆幼苗低温胁迫下的生理响应及耐寒性鉴定

Physiological Response to Cold Stress and Identification of Its Cold Tolerance in Different Pigeonpea Seedling Stage

木豆[Cajanus cajan (L.) Millsp.]为热区重要的粮饲兼用作物。为解析木豆响应低温胁迫生理特性及耐寒能力,本研究以8份木豆种质幼苗为试验材料,通过低温胁迫(4℃)处理,对木豆8个生理指标测定分析,运用隶属函数综合分析法鉴定其耐寒性。结果表明:随低温胁迫时间的延长,木豆相对电导率(REC)和可溶性糖(SS)、丙二醛(MDA)、脯氨酸(Pro)的含量呈升高趋势,超氧化物气化酶(SOD)、过氧化物酶(POD)活性随低温处理时间的延长呈先升后降之势,叶绿素(Chl)含量总体呈下降趋,过氧化氢酶(CAT)活性变化存在差异,仅P_(2)中CAT活性差异显著。运用隶属函数综合评价木豆耐寒性强弱为:缅甸木豆>海南儋州木豆>木豆(170306001)>木豆(060302216)>ICPL7086>木豆(南02137)>广西木豆>海南琼中木豆,且木豆耐寒性与Chl含量、SOD和POD活性呈显著相关,这些指标可以作为木豆耐寒性评价的可信指标。

Pigeonpea is an important food and forage crop in the tropics.In order to dissect the physiological character-istics of pigeonpea under cold stress and its cold tolerance,eight seedlings of pigeonpea were tested and analyzed by 8 physiological indexes under 4℃stress,and cold tolerance of pigeonpea were evaluated by subordinate function value method in this study.The results showed that with the prolongation explored to cold stress,REC,SS,MDA and Pro increased respectively,SOD and POD increased firstly and then decreased,and Chl showed a general trend of decline,and the variation of CAT activity was significant difference only in P2 among the 8 accessions.The cold tolerance of the 8 accessions was in the order:Miandian>Danzhou>170306001>060302216>ICPL7086>Nan 02137>Guangxi>Qiong-zhong.The content of Chl,SOD and POD activity were significantly correlated with cold tolerance of pigeonpea,which could be the reliable physiological indexes to evaluate the cold tolerance of pigeonpea.