小鼠生物净化中体外受精试剂对其受精率和产仔率的影响

Effect of In Vitro Fertilization Reagent on Fertilization Rate and Birth Rate of Mice in Biological Purification

目的在基因修饰小鼠的生物净化中探索体外受精试剂对受精率和产仔率的影响。方法根据体外受精试剂厂家不同,实验体系分为A组、B组和C组。分别选择5~11种基因修饰小鼠品系进行超数排卵-体外受精-胚胎移植实验,记录捡卵数、2细胞数、移植胚胎数、仔鼠出生数等指标,并计算受精率和产仔率。结果A组的受精率为(51.89±13.91)%,产仔率为(14.70±10.95)%;B组的受精率为(57.71±17.12)%,产仔率为(34.96±9.92)%;C组的受精率为(54.78±18.56)%,产仔率为(22.01±9.54)%。受精率:B>C>A,产仔率:B>C>A,但各组之间无统计学差异。A组所需试剂配制难度大、使用复杂、稳定性较差,而B组和C组试剂使用简单、稳定性强。但与A组和C组相比,B组所使用的试剂单价高,因此实验成本较高。结论B组和C组的试剂均可作为基因修饰小鼠体外受精的常规试剂使用,实验人员可根据受精率、产仔率、价格和使用简便性等综合因素进行选择。

Objective To investigate the effects of IVF reagents on the fertility rate and birth rate of genetically modified mice in biological purification.Method The experimental system was divided into group A,group B and group C according to different manufacturers of IVF reagents.5-11 genetically modified mouse strains were selected for superovulation,IVF-ET experiments,and the number of eggs collected,the number of 2-cell,the number of embryos transferred,and the number of piglets born were recorded,and the fertilization rate and the birth rate were calculated.Result The fertilization rate of group A was(51.89±13.91)%and the puerperal rate was(14.70±10.95)%.In group B,the fertilization rate was(57.71±17.12)%and the birth rate was(34.96±9.92)%.In group C,the fertilization rate was(54.78±18.56)%and the puerperal rate was(22.01±9.54)%.Fertilization rate:B>C>A;Puerperal rate:B>C>A,but there was no statistical difference among all groups.The reagents required by group A are difficult to prepare,complicated to use and have poor stability,while the reagents of group B and group C are simple to use and have strong stability.However,compared with group A and group C,the unit price of reagents used in group B is high,so the experimental cost is higher.Conclusion The reagents of group B and group C can be used as conventional reagents for IVF of genetically modified mice,and the experimental can choose them according to the comprehensive factors such as fertilization rate,puerperal rate,price and ease of use.