推拿对阿片类药物性便秘大鼠TRPV1、β-arrestin2、MOR的影响

Effects of Tuina on TRPV1,β-arrestin2 and MOR in opioid-induced constipation rats

目的:验证推拿对阿片类药物性便秘(OIC)的干预作用,观察推拿对OIC大鼠模型肠道组织中瞬时受体电位香草酸1(TRPV1)、β-抑制蛋白2(β-arrestin2)、μ-阿片受体(MOR)的影响。方法:将50只SD大鼠随机分为空白组、OIC组、OIC+推拿组、TRPV1阻断剂组、OIC+TRPV1阻断剂+推拿组,每组10只。采用吗啡持续注射制备OIC动物模型,通过顺时针摩腹进行干预,观察其干预作用,Western Blot、RT-PCR检测肠道组织中TRPV1、β-arrestin2、MOR蛋白及mRNA的表达。结果:与OIC组比较,OIC+推拿组大鼠粪便颗粒数、粪便湿重、粪便含水量均显著改善(P<0.05,P<0.01);推拿可以上调OIC大鼠肠道组织中TRPV1的表达(P<0.05);OIC+推拿+TRPV1阻断剂组的大鼠粪便颗粒数及粪便含水量增加(P<0.05),但粪便湿重无统计学意义。结论:推拿可以缓解OIC;推拿对OIC动物模型肠道组织中TRPV1、β-arrestin2、MOR的调控机制仍需进一步深入探索。

Objective:To verify the intervention effects of Tuina on opioid-induced constipation(OIC),to observe the effects of Tuina on the TRPV1,β-arrestin2,andμ-opioid receptors(MDR)in intestinal tissue of OIC rat model.Methods:Fifty SD rats were randomly divided into blank group,OIC group,OIC+Tuina group,TRPV1 blocker group,OIC+TRPV1 blocker+Tuina group,10 rats in each group.The OIC rat model was prepared by continuous injection of Morphine,and the intervention was performed by rubbing the abdomen clockwise to observe its intervention effect.The protein and mRNA expressions of TRPV1,β-arrestin2 and MOR in intestinal tissues were detected by Western Blot and RT-PCR.Results:Compared with the OIC group,the number of feces particles,wet weight of feces,and water content of feces in the OIC+Tuina group were significantly improved(P<0.05,P<0.01);Tuina could up-regulate the expression of TRPV1 in the intestinal tissue of OIC rat model(P<0.05),the number of feces particles and water content in feces of rats in the OIC+Tuina+TRPV1 blocker group increased(P<0.05),but the wet weight of feces was not statistically significant.Conclusion:Tuina can relieve OIC;the regulatory mechanism of Tuina on TRPV1,β-arrestin2,and MOR in the intestinal tissue of OIC rat model still needs furtherexploration.