超声辅助深共熔溶剂提取栀子黄酮及其对HepG2细胞的降血糖作用

Extraction of Gardenia flavone with ultrasound-assisted deep eutectic solvents and its hypoglycemic effect on HepG2 cells

采用超声辅助深共熔溶剂(DES)对栀子黄酮进行了提取,通过单因素实验、正交实验优化了提取工艺,测试了提取的栀子黄酮溶液对人肝脏胚胎肿瘤细胞(HepG2细胞)的降血糖能力。结果表明,DES含水量(以加水前DES的体积计,下同)对黄酮得率的影响最大。在以n(氯化胆碱)∶n(1,2-丙二醇)=1∶3制备的DES为提取溶剂、含水量30%、料液比(g∶m L,下同)为1∶33、超声(功率120 W、时间40 min、温度60℃)的最佳工艺条件下,栀子黄酮得率最高,为32.70 mg/g。中(250.0 mg/L)、高(1000.0 mg/L)质量浓度的栀子黄酮可显著降低甘油三酯含量。栀子黄酮可通过增加IR-HepG2细胞内己糖激酶、丙酮酸激酶含量,降低甘油三酯的含量而促进糖代谢,从而达到降血糖效果。

Ultrasound-assisted deep eutectic solvent(DES)was used for extraction of Gardenia flavone,with the extraction process optimized by single factor experiments and orthogonal experiments.The Gardenia flavone obtained was then evaluated for its hypoglycemic effect on human hepatocellular carcinomas(HepG2 cells).The results showed that the water content(based on the volume of DES before adding water,the same below)in DES exhibited the greatest influence on the flavone yield.Under the optimum conditions of n(choline chloride)∶n(1,2-propylene glycol)=1∶3 as extraction solvent,water content 30%,solid-liquid ratio(g∶mL,the same below)1∶33,ultrasound conditions(power 120 W,time 40 min,temperature 60℃),the yield of Gardenia flavone reached the highest(32.70 mg/g).Medium(250.0 mg/L)and high(1000.0 mg/L)mass concentrations of Gardenia flavone could significantly reduce the content of triglyceride.Gardenia flavone could promote glucose metabolism by increasing the content of hexokinase and pyruvate kinase in IR-HepG2 cells,reducing the content of triglyceride,and thus achieving hypoglycemic effect.